长臂猿感染H9N2亚型禽流感病毒病例报告

A Case Report of Gibbon Infection with H9N2 Avian Influenza Virus

2022年1月,某动物园同一馆舍内饲养的6只长臂猿(Nomascus spp.)先后出现流清涕、咳嗽的症状。采集患病长臂猿鼻拭子共6份,利用高通量测序法对样本进行病原筛查,结果显示:2份样本呈AIV阳性,序列组装结果与H9N2亚型禽流感病毒的相似性最高;采用qPCR法对2份阳性样本进行H1~H16基因和N1~N9基因检测验证,发现5号样本H9 Ct值为31.35、N2 Ct值为36.16,6号样本H9 Ct值为27.46、N2 Ct值为29.57,均为阳性。给予患病长臂猿化痰止咳、消炎和控制继发感染等综合治疗,同时加强饲养环境消毒,患病长臂猿很快康复。

In January 2022,six gibbons(Nomascus spp.)in the same enclosure of a zoo successively showed symptoms of runny nose and cough.We separately collected six nasal swabs from the six gibbons,detected the pathogens in the samples by high-throughput sequencing technology.The results showed that two samples were positive for AIV.The se⁃quence assembly was similar to H9N2 avian influenza virus.The two positive samples were tested by qPCR for H1-H16 genes and N1-N9 genes and the results showed that Ct values of H9 and N2 in sample 5 were 31.35 and 36.16 respec⁃tively,Ct values of H9 and N2 in sample 6 were 27.46 and 29.57.The gibbons were given comprehensive treatment,such as expectoration and cough relief,anti-inflammatory and secondary infection control,and disinfection of the feeding environ⁃ment was strengthened.The sick gibbons recovered quickly.