香椿子果瓣和中轴对果蝇热耐受能力的影响

Effect of Toona Sinensis Fruit Peel and Axial Tissue on Heat Tolerance of Drosophila Melanogaster

为了研究香椿子果瓣和中轴对黑腹果蝇热耐受能力的影响,试验以野生型黑腹果蝇为研究对象,观察不同浓度香椿子果瓣和中轴玉米琼脂培养基(0、0.1、1、5和10 g/L)对38±1℃热暴露果蝇存活时间的影响,选取提高果蝇热耐受能力最佳的香椿子果瓣组雌果蝇进行热锻炼和运动能力试验。结果显示:香椿子果瓣和中轴不同程度地延长了热暴露果蝇的半数致死时间(LT_(50))、平均存活时间(T)、最高存活时间(T_(max)),果瓣的作用效果最佳以及雌蝇延寿率高于雄蝇;香椿子果瓣显著改善了热锻炼后雌蝇的存活时间和运动能力,随着香椿子果瓣浓度的增大,呈先增后降的趋势且均在浓度为5 g/L时达到最大(P<0.05),说明香椿子果瓣具有提高机体热耐受能力的作用。

To explore the effect of Toona sinensis fruit peel and axial tissue on the tolerance of heat shock on Drosophila melanogaster.Fruit flies were fed with normal corn agar medium containing 0.1,1,5 and 10 g/L of fruit peel and axis powder,respectively,and their survival time was observed at 38±1℃when exposed to 38±1℃.The fruit peel was the optimum material with heat tolerance on female fruit flies.Therefore,they were selected for the heat adaptation and athletic ability test.The results showed that the lethal time of 50%(LT_(50)),mean survival time(T)and maximum survival time(T_(max))of fruit flies were prolonged by fruit peel and axis.The fruit peel displayed the best effect on improving the thermal tolerance of female fruit flies.The fruit peel significantly improved the survival time and motility of female fruit flies after heat exercise.With the increasing concentration of fruit peel,it showed a trend of increasing and decreasing,and reached its maximum at a concentration of 5 g/L(P<0.05),indicating that T.sinensis fruit peel have the function of improving the body s heat tolerance.