响应面法优化亳白芷粗多糖大孔树脂脱色工艺及抗氧化活性研究

Optimization of Decolorization Process and Antioxidant Activity of Crude Polysaccharides from Bo Baizhi Using Response Surface Macroporous Resin Method

为了研究亳白芷粗多糖大孔树脂脱色工艺及其抗氧化性,以脱色率和多糖保留率的综合评分为指标,采用响应面法设计实验,优化亳白芷粗多糖大孔树脂脱色工艺;并对DPPH、羟基自由基的清除能力进行测定,研究亳白芷粗多糖脱色前后抗氧化活性。结果显示亳白芷粗多糖脱色工艺最佳参数为:粗多糖与D-301大孔树脂湿重的质量比为1∶31、脱色温度60℃、脱色时间3.2 h、脱色PH值7.3,最终多糖脱色率为89.03%、保留率为82.14%,综合评分为85.59%;多糖脱色后DPPH和羟基自由基清除率略低于脱色前,且浓度分别在高于4.0mg/mL和3.0mg/mL时接近于VC阳性对照组。结论D301型大孔树脂脱色工艺稳定性、可行性强,可应用于实际生产研究;脱色后亳白芷多糖具有较好的抗氧化活性,可作为天然抗氧化剂。

In order to study the decolorization process and antioxidant activity of Bozhong crude polysaccharide macroporous resin.Using the comprehensive evaluation of decolorization rate and polysaccharide retention rate as indicators,an experiment was designed using response surface methodology to optimize the decolorization process of crude polysaccharide macroporous resin from Radix Angelicae Dahuricae;And the scavenging ability of DPPH and hydroxyl radicals was measured to study the antioxidant activity of crude polysaccharides from Angelica dahurica before and after decolorization.The results showed that the optimal parameters for the decolorization process of crude polysaccharides from Angelica dahurica were:the mass ratio of crude polysaccharides to D-301 macroporous resin wet weight was 1:31,decolorization temperature was 60℃,decolorization time was 3.2h,decolorization pH value was 7.3,and the final decolorization rate of polysaccharides was 89.03%,retention rate was 82.14%,and comprehensive score was 85.59%;After decolorization,the DPPH and hydroxyl radical scavenging rates of polysaccharides were slightly lower than before decolorization,and the concentrations were close to the VC positive control group when they were higher than 4.0mg/mL and 3.0mg/mL,respectively.Conclusion:The D301 macroporous resin decolorization process has strong stability and feasibility,and can be applied to practical production research;After decolorization,polysaccharides from Baizhi have good antioxidant activity and can be used as natural antioxidants.