Genome Sequencing,Probiotic Analysis,and Oxalate Degradation Modification of Limosilactobacillus reuteri Q35

罗伊氏乳杆菌Q35的基因组测序、益生性分析和草酸降解能力改造

Limosilactobacillus reuteri is a microbe intricately linked to humans and animal health.A thorough assessment of its safety and potential benefits is imperative prior to its application in human and animals.In this investigation,we performed a comprehensive analysis encompassing genome sequencing,genomic analysis,and phenotypic characterization of L.reuteri Q35,an exceptionally proficient producer of reuterin.The whole genome sequencing results showed that the complete genome sequence spans 2145158 bp with a GC content of 38.9%and encompasses 2121 genes.Initial identification of antibiotic-resistant genes,virulence factors,and toxin-coding genes in the genome substantiated the strain’s low-risk status.Subsequent tests for antibiotic resistance,acute oral toxicology,and hemolysis further confirmed its elevated safety level.The genome of L.reuteri Q35 was found to contain genes associated with adhesion and stress tolerance.Following exposure to artificial gastric juice and bile salt,the strain exhibited a higher survival rate and demonstrated a strong scavenging ability for hydroxyl free radicals in antioxidant capacity tests.These findings suggested that L.reuteri Q35 possesses unique probiotic properties.Additionally,the genome of strain Q35 harbors three truncated oxaloyl-CoA decarboxylase genes(oxc1,oxc2 and oxc3),overexpression of which resulted in a significant increase in ammonium oxalate degradation from 29.5%to 48.8%.These findings highlight that L.reuteri Q35 exhibits both favorable safety characteristics alongside beneficial properties,making it a promising candidate for treating metabolic disorders such as hyperoxaluria.

罗伊氏乳杆菌(Limosilactobacillus reuteri)是一类与人类和动物机体健康密切相关的微生物,然而菌株应用于人类和动物前需要对其安全性和益生性进行评估。本研究旨在对一株高产罗伊菌素的罗伊氏乳杆菌Q35进行了全基因组测序、益生性分析和草酸降解能力改造。全基因组测序结果显示,该菌株完整基因组序列长度为2145158 bp,GC含量为38.91%,有2121个基因。本文对基因组中抗生素抗性、毒力因子和毒素编码基因进行了鉴定和分析,并通过抗生素耐药性、急性口服毒理学和溶血测试等研究证实,该菌株具有较高的安全水平。基因组上发现粘附和应激耐受相关基因的存在,经人工胃液和胆汁盐胁迫后菌株存活率较高,抗氧化能力测试结果显示,该菌株对羟自由基具有较强的清除能力。以上结果表明,罗伊氏乳杆菌Q35具有特殊的益生菌特性。此外,本文在菌株Q35基因组上鉴定到3个短的草酰辅酶A脱羧酶基因oxc1、oxc 2和oxc 3,通过过量表达这3个基因使菌株降解草酸铵的能力由29.5%提高到48.8%。本研究结果表明,罗伊氏乳杆菌Q35具有良好的安全性和益生性,该菌株在高草酸尿症等代谢疾病的治疗中具有一定的应用潜力。