川续断浸泡液条件优化及其抗氧化性试验研究

Experimental Study on Condition Optimization and Oxidation Resistance of Dipsacus asper Soaking Solution

为开发川续断代茶饮,以川续断皂苷Ⅵ溶出量为质量指标,利用高效液相色谱法研究了盐续断浸泡条件,考察温度、时间、料液比、续断粒度4个因素对川续断皂苷Ⅵ溶出量的影响,并应用响应曲面(Box-Benhnken)设计优化浸泡的条件。同时,利用紫外可见分光光度计,以DPPH自由基清除法和ABTS^(+)自由基清除法研究浸泡液的抗氧化活性。结果表明,盐续断的最佳浸泡条件为浸泡温度100℃,液料比值55 mL/g,颗粒目数0.250 mm,浸泡时间36 min,此条件下川续断皂苷Ⅵ的溶出量为67.424 mg/g;盐续断浸泡液具有良好的抗氧化活性,其对DPPH·的IC_(50)值为0.50 mg/mL,对ABTS^(+)·的IC_(50)值为0.23 mg/mL;当质量浓度为0.728 mg/mL时,其对ABTS^(+)·的清除率达100%。

To develop a Dipsacus asper tea substitute using Dipsacus asper(salt-processed),this study employed the dissolution of AsperosaponinⅥas a quality indicator,investigating the soaking conditions of salt processing.The effects of temperature,time,liquid-to-material ratio,and particle size on the dissolution of AsperosaponinⅥwere examined,and the soaking conditions were optimized using response surface methodology(Box-Behnken design).Additionally,the antioxidant activity of the soaking solution was studied using UV-visible spectrophotometry with DPPH·and ABTS^(+)·assays.The results indicated that the optimal soaking conditions for salt processing were a soaking temperature of 100℃,a liquid-to-material ratio value of 55 mL/g,a particle size of 0.250 mm,and a soaking time of 36 minutes,resulting in a AsperosaponinⅥdissolution of 67.424 mg/g.The soaking solution exhibited good antioxidant activity,with IC_(50)values of 0.50 mg/mL for DPPH·and 0.23 mg/mL for ABTS^(+).When the mass concentration was 0.728 mg/mL,the clearance rate of ABTS^(+)·reached 100%.