微波辅助提取毛竹叶黄酮及其抗氧化活性的研究

Study on Microwave-Assisted Extraction of Flavonoids and Antioxidant Activities from Phyllostachys Edulis Leaves

以毛竹叶为原料,在单因素试验的基础上,结合响应面分析法对微波辅助提取毛竹叶黄酮的工艺参数进行优化,并对毛竹叶黄酮进行体外抗氧化试验。结果显示:微波辅助提取毛竹叶黄酮的最佳工艺参数为液料比29∶1(mL/g)、乙醇体积分数70%、微波功率300 W、微波时间300 s,该条件下毛竹叶黄酮提取得率达1.9205%;体外抗氧化结果表明,毛竹叶黄酮对超氧阴离子、羟自由基和过氧化氢均具有清除作用,其中对过氧化氢的清除能力要显著高于维生素C和BHT,而对超氧阴离子和羟自由基的清除能力低于维生素C但均高于BHT。

In this paper,phyllostachys edulis leaves were used as raw material.On the basis of single factor experiment and response surface analysis,the process parameters of microwave-assisted extraction of flavonoids from phyllostachys edulis leaves were optimized,and the antioxidant experiment of flavonoids from phyllostachys edulis leaves was carried out in vitro.The results showed that the optimum technological parameters for microwave-assisted extraction of flavonoids from leaves of phyllostachys edulis were as follows:liquid-material ratio 29∶1(mL/g),ethanol concentration 70%,microwave power 300 W,and microwave time 300 s,and the extraction rate of flavonoids from leaves of phyllostachys edulis reached 1.9205%.In vitro antioxidant results showed that flavonoids in leaves of phyllostachys edulis had scavenging effects on superoxide anion,hydroxyl radical and hydrogen peroxide,of which the scavenging ability of superoxide anion and hydroxyl radical was significantly higher than that of vitamin C and BHT,while the scavenging ability of superoxide anion and hydroxyl radical was lower than that of vitamin C but higher than that of BHT.