2022年云南省柯萨奇B组5型病毒全基因组分析

Whole-genome analysis of coxsackievirus B5 isolated in Yunnan province,China in 2022

目的分析2022年云南省4株柯萨奇B组5型病毒(CVB5)分离株的全基因组特征。方法设计CVB5全基因组扩增及测序引物,分段扩增CVB5分离株的全基因组,采用“引物移步法”测序,并进行序列拼接和组装。采用MEGA 7.0、Simplot 3.5.1和DNAStar 7.1等软件对CVB5毒株的全基因组序列进行遗传特征分析。结果基于VP1和全基因组系统进化分析发现,4株CVB5分离株均为C基因型。与原型株相比,4株毒株VP1区均发生多处氨基酸变异。P1、P2、P3系统进化分析和simplot重组分析显示,CVB5分离株可能与多种肠道病毒B群毒株发生重组。结论2022年云南省昆明地区手足口病(HFMD)患者临床样本中分离到的4株CVB5均属于C基因型。

Objective To analyze the whole-genome characteristics of four coxsackievirus B5(CVB5)strains isolated in Yunnan province,China in 2022.Methods Primers were designed to amplify and sequence the whole genome of CVB5 isolates.The whole genome was amplified in segments,and sequenced using the"primer walking"method.Sequence splicing and assembly were performed.Genetic characteristics of the CVB5 strains were analyzed using software such as MEGA 7.0,Simplot 3.5.1,and DNAStar 7.1.Results Based on VP1 and whole-genome phylogenetic analysis,all four CVB5 isolates belonged to genotype C.Compared with the prototype strain,multiple amino acid mutations occurred in the VP1 region of the four strains.Phylogenetic analysis of P1,P2,and P3 regions and Simplot recombination analysis showed that the CVB5 isolates in this study may have undergone recombination with multiple enterovirus B strains.Conclusion The four CVB5 strains isolated from clinical samples of hand,foot and mouth disease(HFMD)patients in Kunming,Yunnan province in 2022 all belonged to genotype C.