利用高密度遗传图谱定位两种不同环境的水稻剑叶形态性状QTL差异

The Difference of QTL for Morphological Traits of Rice Flag Leaf by High-density Genetic Map in Two Different Environments

水稻剑叶形态性状是构成理想株型的重要因素之一,发掘新的控制剑叶形态性状的基因资源是提高水稻产量的重要途经。本研究利用高密度遗传图谱对在杭州和海南陵水种植的印度粳稻品种M494和中国籼稻品种中9B(Z9B)杂交衍生的重组自交系(recombinant inbred lines,RIL)群体剑叶长、剑叶宽、剑叶面积和剑叶长宽比进行数量性状位点(Quantitative trait locus,QTL)定位检测。研究共检测到10个QTL,包括1个剑叶长QTL、4个剑叶宽QTL、4个剑叶面积QTL和1个剑叶长宽比QTL,分布于第4、第7、第8、第9和第10号染色体,贡献率介于6.47%~13.28%之间;鉴定到2个多效性QTL区间,其中在第4号染色体Block536~Block544区间检测到同时控制剑叶宽和剑叶面积的QTL,在第10号染色体Block942~Block961区间检测到同时控制剑叶宽和剑叶长宽比的QTL,其贡献率分别为8.82%、13.28%、12.28%和12.60%。两地间QTL差异较大,仅在Block536~Block544区间同时定位到控制剑叶宽及剑叶面积的QTL及在Block942~Block961区间同时定位到控制剑叶宽及剑叶长宽比的QTL。

The flag leaf morphological traits are one of the important factors that constitute the ideal plant type in rice.Exploring new genetic resources that control the flag leaf morphological traits is one of the important ways to increase rice yield.In this study,quantitative trait loci(QTLs)were detected for flag leaf length(FLL),flag leaf width(FLW),flag leaf area(FLA),and flag leaf length to width(FLLW)of a population of recombinant inbred lines(RIL)derived from a cross between the Indian japonica rice variety M494 and China indica variety Zhong 9B(Z9B)planted at Hangzhou and Hainan using high-density genetic map.A total of 10 QTL were detected including one QTL for flag leaf length,four QTL for flag leaf width,four QTL for flag leaf area and one QTL for flag leaf aspect ratio,distributed on chromosomes 4,7,8,9 and 10,which could explain 6.47%-13.28%of the phenotypic variation.Two pleiotropic QTL intervals were identified,and the locus was located between Block536 and Block544 on chromosome 4 which were simultaneously controlled FLW and FLA,and the locus between Block942 and Block961 on chromosome 10 simultaneously controlling FLW and FLLW,with phenotypic variation were 8.82%,13.28%,12.28%and 12.60%,respectively.The QTL varied greatly between the two sites,with QTL controlling flag leaf width and flag leaf area only simultaneously position in Block536-Block544 and QTL controlling flag leaf width and flag leaf length to width only in the Block942-Block961 interval.