蜜瓜籽不同溶剂提取物活性成分及抗氧化能力分析

Active ingredients and antioxidant capacity analysis of different solvent extracts from melon seeds

以河套地区5个不同品种蜜瓜籽(华莱士、银蜜、西州密25号、玉妃和红蜜)为研究对象,分别采用蒸馏水、70%乙醇、70%丙酮等不同溶剂进行提取,测定其总黄酮、总多酚和总多糖含量并与抗氧化活性进行相关性分析。结果表明,不同溶剂提取物抗氧化效果不同,70%乙醇提取物清除DPPH自由基、ABTS+自由基和抗氧化还原能力最强;华莱士瓜籽70%乙醇提取物中总黄酮和总多酚含量(w,后同)最高,分别为94.74、35.90 mg·g^(-1);总多糖含量最高的是华莱士瓜籽水提取物,达159.29 mg·g^(-1)。相关性分析表明,总多酚含量与DPPH自由基清除能力、ABTS+自由基能力和还原能力呈显著或极显著正相关,总黄酮含量与DPPH自由基清除能力、还原能力呈显著或极显著正相关。建议将70%乙醇作为河套蜜瓜籽的提取溶剂,为河套蜜瓜副产物的进一步开发、提取和利用提供理论依据。

In this study,distilled water,70%ethanol and 70%acetone respectively were used as solvent to extract flavo-noids,polyphenols and polysaccharides from five different cultivars of melon seeds(Hualaishi,Yinmi,Xizhoumi,Yufei and Hongmi).The correlation analysis between bioactive components and their antioxidant ability were explored.The results showed that fractions extracted by 70%ethanol from all samples expressed the best anti-redox capacity by significantly scavenging DPPH and ABTS+radicals.Moreover,fractions extracted by 70%ethanol from Hualaishi melon seeds had the highest amount of total flavonoids(94.74 mg·g^(-1))and total polyphenols(35.90 mg·g^(-1))and fractions ex-tracted by distilled water from Hualaishi melon seeds had the highest amount of total polysaccharides(159.29 mg·g^(-1)).The results of correlation analysis showed that the amount of total flavonoids and total polyphenols had significant positive correlations with the ability of scavenging DPPH,ABTS+radicals and anti-redox.In conclusion,70%ethanol was a potential preferences for extracting bioactive components from melon seeds and the findings would establish a theoretical basis for further research of by-products produced by Hetao melon.