9份内蒙古大豆种子携带病原菌的检测分析

Detection and analysis of nine servings soybean seed borne pathogens in Inner Mongolia

为明确内蒙古大豆种子内部和表面携带的病原菌种类和分离频率,探究大豆种子是否携带检疫性病原菌大豆疫霉(Phytophthora sojae),采用洗涤检验法和培养基培养法,分别从供试大豆种子内部获得218株真菌分离物,种子表面获得196株真菌分离物,但未分离获得P.sojae。结合菌落形态观察和ITS-rDNA序列分析对分离物进行鉴定,种子内部真菌分离物分属16个属,种子表面真菌分离物分属17个属。结合文献报道从中挑选了9个属的24株疑似病原菌进行致病力测定,发现其均可在大豆黄化苗上引起病斑。通过特异性引物检测法进一步确认了供试的大豆种子未携带大豆疫霉。上述结果为大豆种子带菌所引起病害的科学防治提供了重要参考。

To clarify the pathogen species and isolation frequency inside and outside of soybean seeds in Inner Mongolia,and to make clear whether soybean seeds carry quarantine pathogen Phytophthora sojae,a total of 218 isolates were obtained from the inside of seeds and 196 isolates were obtained from the outside of seeds using washing assay and medial culture method,respectively.P.sojae was not isolated from the soybean seeds tested.Combined with colony morphology observation and ITS-rDNA sequence analysis,the above isolates were preliminarily identified.The internal isolates of seeds belonged to 16 genera,and the external isolates of seeds belonged to 17 genera.In combination with literature reports,24 candidate fungal strains belonging to 9 genera were selected and tested for virulence,it showed that they all could cause lesions on etiolated soybean seedlings.Finally,the tested soybean seeds were confirmed to be free of P.sojae by specific primer amplification.These results provide an important reference for the scientific control of soybean diseases caused by seed borne pathogens.