马铃薯卷叶病毒属病毒在云南的发生分布与种类鉴定

Occurrence,distribution and virus identification of poleroviruses in Yunnan

南方菜豆花叶一品红病毒科(Solemoviridae)的马铃薯卷叶病毒属(Polerovirus)病毒种类繁多,寄主范围广,能侵染豆科、茄科、葫芦科、十字花科等多个科的植物,在全世界造成极大的经济损失,且常与番茄丛矮病毒科(Tombusviridae)的幽影病毒属(Umbravirus)病毒复合侵染引起严重的植物病害。为了探明马铃薯卷叶病毒属病毒(poleroviruses)在云南的发生分布情况,以及poleroviruses与幽影病毒属病毒(umbraviruses)复合侵染可能引发的爆发风险,本研究采用RT-PCR检测方法,对云南的蔬菜、水果等经济作物和作物周边的杂草开展了poleroviruses发生情况调查及病毒种类的检测鉴定。从云南省昆明市、玉溪市、保山市、大理州、楚雄州、西双版纳州和红河州7个州市采集的6科25种的669份蔬菜、烟草、马铃薯、鸡蛋果等经济作物和作物周边杂草样品中,在11种经济作物中检测到6种poleroviruses,分别为马铃薯卷叶病毒(potato leafroll virus,PLRV)、南瓜蚜传黄化病毒(cucurbit aphid-borne yellows virus,CABYV)、丝瓜蚜传黄化病毒(suakwa aphid-borne yellows virus,SABYV)、辣椒脉黄化病毒1号(pepper vein yellows virus 1,PeVYV-1)、辣椒脉黄化病毒3号(pepper vein yellows virus 3,PeVYV-3)和芸薹黄化病毒(brassica yellows virus,BrYV)。其中PeVYV-3的平均检出率最高,为6.73%,是为害云南省蔬菜、水果中poleroviruses的优势种。BrYV侵染豌豆、PeVYV-3侵染茄子、PeVYV-1侵染豌豆和蚕豆、CABYV侵染烟草和豌豆均为国内外首次报道,并且云南首次发现了SABYV的感染。Poleroviruses的寄主范围在逐渐扩大,尤其是在云南各地分布范围较广,这表明poleroviruses对作物的危害风险在逐渐增加。研究结果有助于深入了解云南主要poleroviruses的种类、分布情况以及它们的发生趋势,为综合防控poleroviruses侵染引起的植物病害提供了参考。

Viruses in the genus Polerovirus of the family Solemoviridae exhibit a broad host range and can infect plants from many families,including Fabaceae,Solanaceae,Cucurbitaceae,Brassicaceae and others.They are responsible for significant economic losses globally and frequently co-infect with umbraviruses,which are members of the family Tombusviridae,leading to severe plant diseases.In order to explore the occurrence and distribution of poleroviruses in Yunnan,along with the potential outbreak risk associated with co-infection involving umbraviruses,a comprehensive disease survey was conducted in commercial crops including vegetables and fruits,as well as in the weeds surrounding these crops in Yunnan.Additionally,virus species were also detected and identified by RT-PCR.A total of 669 samples of 5 families,comprising 25 species of commercial crops and surrounding weeds,including vegetables,tobacco,potatoes,passion fruit and others,were collected from 7 states and cities in Yunnan Province,including Kunming,Yuxi,Baoshan,Dali,Chuxiong,Xishuangbanna and Honghe.Among the 11 commercial crops,6 species of poleroviruses were found,which were the species potato leafroll virus,the species cucurbit aphid-borne yellows virus,the species suakwa aphid-borne yellows virus,the species pepper vein yellows virus 1,the species pepper vein yellows virus 3,and the species brassica yellows virus,respectively.Among them,PeVYV-3 had the highest average detection rate of 6.73%and was the dominant virus species in vegetables and fruits in Yunnan province.It was the first report in domestic and abroad that BrYV infected pea,PeVYV-3 infected eggplant,PeVYV-1 infected pea and broad bean,CABYV infected tobacco and pea.Moreover,the occurrence of SABYV in Yunnan Province was first reported.The host range of poleroviruses is gradually expanding,especially in various parts of Yunnan,indicating that the harm of poleroviruses to crops is gradually increasing.In addition,there is a risk of disease outbreaks with umbravirus co-infection.The research results contribute to a deeper understanding of the main types,distribution,and occurrence trends of poleroviruses in Yunnan,providing reference for comprehensive prevention and control of plant diseases caused by poleroviruses and their combined infection with umbraviruses.