多效唑对鸡爪槭生长及叶色的影响

Effects of paclobutrazol on the growth and leaf color of Acer palmatum Thunb.

本研究以江苏省农业科学院休闲农业研究所槭树创新团队自主选育的鸡爪槭新品种金陵丹枫二年生幼苗为试验材料,探究不同质量浓度多效唑对金陵丹枫生长、叶色、叶绿素荧光指标及抗氧化酶活性的影响。在春季鸡爪槭展叶初期设定7个多效唑质量浓度(分别为150 mg/L、300 mg/L、500 mg/L、800 mg/L、1500 mg/L、2000 mg/L、2500 mg/L)整株喷施。结果表明,喷施不同质量浓度多效唑均能明显抑制金陵丹枫植株生长,且多效唑质量浓度越高,金陵丹枫株高越矮、当年生新梢长度越短。同时,喷施多效唑会影响金陵丹枫的叶色,导致叶片变红,花青素含量显著升高。进一步研究结果表明,花青素合成途径关键基因ApPYL、ApCHS、ApCHI、ApF3H和ApDFR的表达量都高于对照。当多效唑质量浓度达到2000 mg/L时,叶片受到明显伤害。本研究中金陵丹枫矮化最适宜的多效唑质量浓度为1500 mg/L。适宜质量浓度多效唑喷施28 d后,金陵丹枫叶片的F_(v)/F_(o)值、F_(v)/F_(m)值,过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和可溶性糖含量显著升高,表明多效唑在一定的质量浓度范围内可提高鸡爪槭金陵丹枫对逆境胁迫的耐受能力,并能对鸡爪槭进行盆栽矮化和提高观赏性。

In this study,we used two-year-old seedlings of Jinling Danfeng,a new variety of Acer palmatum Thunb.independently selected and bred by Jiangsu Academy of Agricultural Sciences,as experimental materials,and investigated the effects of different mass concentrations of paclobutrazol on the growth,leaf color,chlorophyll fluorescence parameters,and antioxidant enzyme activities of Jinling Danfeng.Seven spraying mass concentrations(150 mg/L,300 mg/L,500 mg/L,800 mg/L,1500 mg/L,2000 mg/L,2500 mg/L)were set in the spring at the beginning of leaf development.The results showed that spraying different mass concentrations of paclobutrazol significantly inhibited the growth of A.palmatum Jinling Danfeng,and the higher the spraying mass concentration,the shorter the plant height and the length of the current year’s new shoots.Spraying paclobutrazol affected the leaf color of A.palmatum Jinling Danfeng,resulting in reddening of the leaves and a significant increase in anthocyanin content.The expression levels of key genes ApPYL,ApCHS,ApCHI,ApF3H and ApDFR in the anthocyanin synthesis pathway were higher than those in the control.The leaves were significantly injured when the mass concentration of paclobutrazol reached 2000 mg/L.In this study,the optimum mass concentration of paclobutrazol to promote dwarfing of Jinling Danfeng was 1500 mg/L.The F_(v)/F_(o)value,F_(v)/F_(m)value,peroxidase(POD)activity,superoxide dismutase(SOD)activity,catalase(CAT)activity and soluble sugar content of leaves were also significantly increased after 28 days of spraying with appropriate mass conentration of paclobutrazol,indicating that paclobutrazol could improve the tolerance ability of A.palmatum Jinling Danfeng to adversity stress in a certain mass concentration range,and could dwarf and improve the ornamental value of potted Acer palmatum Thunb.