槲皮素对鹌鹑生产性能、蛋品质及肠道菌群的影响

Effects of quercetin on production performance, egg quality and gut microbiota of quails

为明确日粮中添加槲皮素对鹌鹑生产性能、蛋品质和性激素水平及肠道菌群的影响,促进槲皮素在鹌鹑生产中的应用,将288羽7周龄黄羽产蛋雌鹌鹑随机平均分为4组,分别饲喂基础饲料、添加0.02%槲皮素的基础饲料、添加0.04%槲皮素的基础饲料、添加0.08%槲皮素的基础饲料,饲喂56 d,测定各处理鹌鹑生产性能指标、蛋品质指标、卵巢激素水平,并对鹌鹑粪便进行16S rDNA测序,分析日粮中添加槲皮素对鹌鹑肠道微生物群落的影响。结果表明,添加0.04%槲皮素的日粮处理能够增强鹌鹑的蛋壳强度,添加0.08%槲皮素的日粮处理能够提高鹌鹑的产蛋率、蛋壳比例、蛋壳强度和蛋黄比例,但对平均蛋重、日采食量和料蛋比无显著影响;添加0.08%槲皮素的日粮处理可显著提高鹌鹑卵巢中孕酮(PROG)浓度和降低催乳素(PRL)浓度;添加0.08%槲皮素的日粮处理可降低有害菌群相对丰度。综上,添加0.08%槲皮素的日粮处理可提高鹌鹑生产性能和蛋品质,且槲皮素可降低肠道内有害菌群的相对丰度,促进机体健康。

In order to clarify the effects of quercetin on the production performance,egg quality,sex hormone levels and gut microbiota of quails,and to promote the application of quercetin in quail production,288 seven-week-old yellow-feathered egg-laying female quails were randomly divided into four groups and fed with basal diet,basal diet supplemented with 0.02%quercetin,basal diet supplemented with 0.04%quercetin,and basal diet supplemented with 0.08%quercetin for 56 days.The production performance indices,egg quality indices and ovarian hormone levels of quails were measured,and the feces of quails were sequenced by 16S rDNA.The effects of dietary quercetin on the intestinal microbial community of quails were analyzed.The results showed that the dietary treatment with 0.04%quercetin supplementation could enhance the eggshell strength of quails.The dietary treatment with 0.08%quercetin supplementation could increase the laying rate,eggshell specific gravity,eggshell strength and yolk specific gravity of quails,but had no significant effect on average egg weight,daily feed intake or feed-egg ratio.The diet supplemented with 0.08%quercetin could significantly increase the concentration of progesterone(PROG)and decrease the concentration of prolactin(PRL)in quail ovary.The dietary treatment with 0.08%quercetin supplementation could reduce the relative abundance of harmful microbiota.In summary,dietary treatment with 0.08%quercetin supplementation can improve the production performance and egg quality of quails and reduce the relative abundance of harmful microbiota in the intestine and promote the health of the body.