酶解联合动态高压微射流制备淀粉/百里酚纳米乳液及其结构与性质分析

Structure and Property Analysis of Starch/Thymol Nanoemulsion Prepared by Enzymolysis Combined with Dynamic High Pressure Microfluidization

本文以蜡质玉米淀粉、百里酚为原料,利用酶解联合动态高压微射流技术制备淀粉/百里酚纳米乳液(S-TNE)。通过调整百里酚的添加顺序SDTM(淀粉-动态高压微射流-百里酚-磁力搅拌)、STMD(淀粉+百里酚-磁力搅拌-动态高压微射流)和STDM(淀粉+百里酚-动态高压微射流-磁力搅拌)探讨不同制备方式对S-T-NE多维度结构与性质的影响。结果表明,STMD方式制备的S-T-NE颗粒未发生明显的聚集,百里酚均匀地吸附在纳米淀粉颗粒上,且储存稳定性最好;纳米淀粉对百里酚还具有最高的负载率(78.30%±1.73%)、载药量(223.71±3.02μg/mg);此外,该方式制备的S-T-NE能有效抑制大肠杆菌和金黄色葡萄球菌的生长,抑菌圈直径分别为16.15±0.19 mm和13.98±0.26 mm,同时具有最佳的铁还原能力(1.36±0.04 mmol Trolox/L)和DPPH、ABTS+自由基清除能力(0.11±0.00、0.43±0.00 mmol Trolox/L)。以上结果可进一步为淀粉基纳米乳液负载活性物质的开发和应用中提供技术参考。

In the present work,starch/thymol nano-emulsions(S-T-NE)were prepared by enzymatic hydrolysis combined with dynamic high-pressure microfluidization technology.Three different methods including starch-DHPM-thymolmagnetic stirring(SDTM),starch-thymol-magnetic stirring-DHPM(STMD),and starch-thymol-DHPM-magnetic stirring(STDM)were selected to discuss the multi-dimensional structure and property discrepancies of S-T-NE.Results showed that S-T-NE particles exhibited the minimal aggregation with uniformly adsorption of thymol by STMD,which was benefited to ensure the excellent storage stability of S-T-NE.In the meantime,the S-T-NE prepared by STMD displayed the highest thymol loading rate(78.30%±1.73%)and drug loading capacity(223.71±3.02μg/mg)compared with other methods.Furthermore,the growth of Escherichia coli and Staphylococcus aureus was obviously inhibited by S-T-NE,showing inhibition zone diameters with 16.15±0.19 mm and 13.98±0.26 mm,respectively.For another,the S-T-NE prepared by STMD exhibited remarkable iron reduction capability(1.36±0.04 mmol Trolox/L)and DPPH,ABTS+free radical scavenging abilities(0.11±0.00,0.43±0.00 mmol Trolox/L).These findings provided valuable technical support on the preparation and application of starch-based nanoemulison loaded with bio-active compounds.