精氨酸对口腔变异链球菌临床株生物膜的抑制作用

Inhibitory effect of arginine on biomembranous of clinical isolates of oral Streptococcus mutans

目的:探究精氨酸对变异链球菌临床株生物膜形成的影响。方法:选取变异链球菌临床株和标准株,用不同质量分数的精氨酸(0.75%、1.5%、3.0%、6.0%)处理作为实验组,用等体积的无菌生理盐水处理为对照组。通过结晶紫染色法和刚果红平板法检测精氨酸对变异链球菌生物膜形成和胞外多糖合成能力的影响;并通过糖酵解pH下降法和耐酸试验检测精氨酸对变异链球菌临床株生物膜产酸和耐酸能力的影响。结果:随着精氨酸质量分数的增加,变异链球菌生物膜形成量和胞外多糖含量均明显减少;精氨酸还可以降低变异链球菌临床株生物膜产酸和耐酸能力。结论:精氨酸对变异链球菌临床株生物膜的形成有抑制作用。

Objective:To explore the effect of arginine on biomembranous of clinical isolates of Streptococcus mutans.Methods:The clinical isolates and standard strains of Streptococcus mutans were selected and treated with different mass fractions of arginine(0.75%,1.5%,3.0%,6.0%)as the experimental group,and treated with equal volume of sterile saline as the control group.The effects of arginine on biofilm formation and extracellular polysaccharide synthesis of Streptococcus mutans were detected by crystal violet staining and Congo red dye method.The effect of arginine on the acid production and acid resistance of biofilm formation of Streptococcus mutans was detected by glycolytic pH drop method and acid resistance test.Results:With the increase of arginine mass fraction,the biofilm formation and extracellular polysaccharide content of Streptococcus mutans decreased significantly.Arginine could also reduce the acid production and acid resistance of biomembranous of clinical isolates of Streptococcus mutans.Conclusion:Arginine can inhibit the biofilm formation of clinical isolates of Streptococcus mutans.