肾癌组织中肥大细胞脱颗粒特征及其与血管形成的关系

Degranulation of mast cells in renal carcinoma and the correlation with angiogenesis

目的探讨肾癌组织中肥大细胞脱颗粒特征及其与肿瘤血管形成的关系。方法通过类胰蛋白酶(Tryptase)免疫组织化学染色标记人肾癌组织及癌旁肾组织中的肥大细胞并统计其浸润密度,根据Tryptase在细胞内外的分布判断肥大细胞脱颗粒状态;通过CD31免疫组织化学染色标记血管内皮细胞并计数肿瘤微血管密度。比较肾癌组织与癌旁肾组织中肥大细胞数量、脱颗粒水平差异,分析肾癌组织中肥大细胞脱颗粒水平与其浸润密度及肿瘤微血管密度的关系。结果共纳入125例肾癌组织标本和52例癌旁肾组织标本。Tryptase染色分析发现肾癌组织和癌旁肾组织中肥大细胞密度分别为(2.67±0.22)、(0.63±0.14)个/高倍视野,肥大细胞脱颗粒阳性率分别为70.3%、34.3%,差异有统计学意义(P<0.01)。肥大细胞密度低、中、高三组的肥大细胞脱颗粒阳性率分别为42.1%、64.1%和83.3%。肥大细胞脱颗粒阳性组肿瘤微血管密度明显高于脱颗粒阴性组[(24.18±1.64)个/高倍视野vs.(10.18±1.97)个/高倍视野,P<0.01]。结论肥大细胞在肾癌组织中的脱颗粒水平明显高于癌旁肾组织,且其脱颗粒水平与肥大细胞浸润数量及肿瘤血管形成密切相关。

Objective To investigate the characteristics of mast cell degranulation in renal carcinoma and the correlation with angiogenesis.Methods Mast cells in human renal carcinoma and adjacent tissues were labeled with Tryptase immunohistochemical staining and the infiltration density was calculated.The degranulation level was evaluated according to the distribution of Tryptase inside and outside the mast cells.Vascular endothelial cells were labeled with CD31 immunohistochemical staining and tumor microvascular density was counted.The difference in the number of cells and degranulation level between renal carcinoma and adjacent tissues were compared.The correlations of degranulation with infiltration density and microvessel density were analyzed.Results A total of 125 renal carcinoma tissues and 52 adjacent tissues were enrolled in the study.Tryptase staining showed that mast cell density in renal carcinoma and adjacent tissues was(2.67±0.22)and(0.63±0.14)cells/HPF,respectively;the positive rate of mast cell degranulation was 70.3%and 34.3%(P<0.01),respectively.According to the density of mast cells,renal carcinoma tissues were divided into three groups:low,medium and high.The positive rate of mast cell degranulation in the three groups was 42.1%,64.1%and 83.3%,respectively.Tumor microvessel density in the degranulation positive group was significantly higher than that in the negative group[(24.18±1.64)vs.(10.18±1.97)cells/HPF,P<0.01].Conclusion The level of mast cell degranulation is significantly higher in renal carcinoma than in adjacent tissues,which is associated with infiltration level and angiogenesis.