基于蛋白组学分析口腔鳞状细胞癌患者颈部淋巴结特征与WPOI-5、DOK4表达水平的关系

Cervical Lymph Node Characteristics in Patients with Oral Squamous Cell Carcinoma and Their Relationship with the Expression Levels of WPOI-5 and DOK4 were Analyzed Based on Proteomics

目的通过蛋白组学分析,探讨口腔鳞状细胞癌(OSCC)患者颈部淋巴结特征与最差侵袭模式类型-5统(WPOI-5)和酪氨酸激酶下游蛋白4(DOK4)表达水平的关系,并评估其在诊断和预后中的价值。方法选取喀什地区第二人民医院2022年1月—2023年2月收治的90例OSCC患者为研究对象,按淋巴结有无受累情况分为转移组(n=44)和无转移组(n=46)。对比两组的临床指标、淋巴结特征指标、生物标记指标及预后指标,并分析WPOI-5和DOK4表达水平与淋巴结转移的关系。结果两组肿瘤位置、肿瘤大小及TNM分期比较,差异无统计学意义(P>0.05);转移组淋巴结大小、淋巴结触诊硬度及淋巴结活动性评分均高于无转移组,淋巴结转移数量多于无转移组,组间差异有统计学意义(P<0.05);转移组WPOI-5表达水平高于无转移组,DOK4蛋白表达水平低于无转移组,组间差异有统计学意义(P<0.05);转移组整体生存、无病生存和平均复发时间均短于无转移组,肿瘤复发率高于无转移组,组间差异有统计学意义(P<0.05);淋巴结转移情况与WPOI-5和DOK4水平呈正相关,差异有统计学意义(P<0.05)。结论颈部淋巴结的特征,如大小、硬度和活动性,以及WPOI-5和DOK4蛋白的表达水平,与OSCC患者的淋巴结转移情况密切相关。淋巴结转移对患者的预后产生了显著影响,导致整体生存率和无病生存期下降。因此,这些指标有助于评估OSCC患者的病情和预后,并成为潜在的治疗靶点。

Objective Exploring the relationship between cervical lymph node characteristics and the expression levels of worst pattern of invasion type-5(WPOI-5)and downstream of tyrosine kinase/docking protein 4(DOK4)in patients with oral squamous cell carcinoma(OSCC)through proteomic analysis.Methods A total of 90 patients with OSCC admitted to the Second People's Hospital of Kashgar from January 2022 to February 2023 were selected as the research objects,according to the presence or absence of lymph node involvement,they were divided into a metastasis group(n=44)and a non-metastasis group(n=46).The clinical indicators,lymph node characteristics,biomarkers and prognostic indicators of the two groups were compared,and the relationship between the expression levels of WPOI-5 and DOK4 and lymph node metastasis was analyzed.Results There was no significant difference in tumor location,tumor size and TNM stage between the two groups(P>0.05);the lymph node size,lymph node palpation hardness and lymph node activity score in the metastasis group were higher than those in the non-metastasis group,and the number of lymph node metastasis was higher than that in the non-metastasis group,the differences between the groups were statistically significant(P<0.05);the expression level of WPOI-5 in the metastasis group was higher than that in the non-metastasis group,and the expression level of DOK4 protein in the metastasis group was lower than that in the non-metastasis group,and the differences between the groups were statistically significant(P<0.05);the overall survival,disease-free survival and average recurrence time of the metastasis group were shorter than those of the non-metastasis group,and the tumor recurrence rate was higher than that of the non-metastasis group,and the differences between the groups were statistically significant(P<0.05);lymph node metastasis was positively correlated with WPOI-5 and DOK4 levels,and the difference was statistically significant(P<0.05).Conclusion The characteristics of cervical lymph nodes,such as size,stiffness,and mobility,as well as the expression levels of WPOI-5 and DOK4 proteins,were closely related to lymph node metastasis in patients with OSCC.Lymph node metastasis has a significant impact on the prognosis of patients,resulting in decreased overall survival and disease-free survival.Therefore,these indicators can help to evaluate the condition and prognosis of OSCC patients and become potential therapeutic targets.