复方苦参膏对湿疹小鼠模型的治疗作用研究

Interventional Effect of Compound Kushen Ointment on Eczema Mouse Model

目的:评价复方苦参膏治疗湿疹的疗效,并探讨其初步作用机制。方法:将56只雄性Kunming小鼠按随机数字法随机分为空白组、模型组、空白基质组、阳性药组(复方醋酸地塞米松乳膏,0.75 mg/cm^(2))、复方苦参膏高剂量组(500 mg/cm^(2))、中剂量组(250 mg/cm^(2))以及低剂量组(125 mg/cm^(2)),每组8只。除空白组外,其余组均用2,4-二硝基氯苯(DNCB)致敏,建立湿疹小鼠模型,激发当天开始给药干预,连续给药14 d,末次给药24 h后取样。对小鼠皮损部位评估打分,计算胸腺和脾脏指数,测定耳肿胀度,苏木精-伊红(HE)染色观察组织病理变化,甲苯胺蓝染色观察肥大细胞情况,酶联免疫吸附试验检测小鼠血清中的白细胞介素-4(IL-4)、IL-5、IL-10、肿瘤坏死因子-(TNF-α)、γ干扰素(IFN-γ)水平的变化。结果:与模型组比较,复方苦参膏各剂量组皮损、耳肿胀度情况均不同程度减轻,表皮厚度变薄,肥大细胞数目明显减少,脾脏和胸腺指数降低,湿疹症状得到缓解,血清中的IL-4、IL-5、IL-10、TNF-α、IFN-γ水平降低,差异有统计学意义(P<0.01,P<0.05)。结论:复方苦参膏对湿疹小鼠有明显的治疗作用,其作用机制可能与调节炎症介质水平,抑制炎症反应,进而维持辅助性T细胞1(Th1)/辅助性T细胞2(Th2)稳态相关。

Objective:To evaluate the efficacy of Compound Kushen Ointment in treating eczema and to explore its preliminary mechanism of action.Methods:Fifty-six male Kunming mice were randomly divided using a random number method into blank control group,model group,blank matrix group,positive drug group(Compound Dexamethasone Acetate Cream,0.75 mg/cm^(2)),and high-,medium-,and low-dose Compound Kushen Ointment groups(500,250,and 125 mg/cm^(2)),with eight mice in each group.Except for the blank control group,all other groups were sensitized with 2,4-dinitrochlorobenzene(DNCB)to establish an eczema mouse model.On the day of induction,treatment was initiated and continued for 14 days.Samples were collected 24 hours after the final administration.The skin lesions of the mice were scored.The thymus and spleen indices were calculated.Ear swelling was measured.Histopathological changes were observed via hematoxylin-eosin(HE)staining.Mast cells were examined using toluidine blue staining,and serum levels of interleukin-4(IL-4),IL-5,IL-10,tumor necrosis factor-α(TNF-α),and interferon-γ(IFN-γ)were assessed using enzyme-linked immunosorbent assay(ELISA).Results:Compared to the model group,all dosage groups of Compound Kushen Ointment showed varying degrees of reduction in skin lesions and ear swelling,along with thinning of the epidermis,a significant reduction in the number of mast cells,and decreased thymus and spleen indices.Eczema symptoms were alleviated,and serum levels of IL-4,IL-5,IL-10,TNF-α,and IFN-γwere significantly reduced(P<0.01,P<0.05).Conclusion:Compound Kushen Ointment has a significant therapeutic effect on eczema in mice,and its mechanism of action may be related to regulating inflammatory mediators,inhibiting inflammatory responses,and maintaining the balance of T-helper cell 1(Th1)/T-helper cell 2(Th2)homeostasis.