巴戟天多糖调控精索静脉曲张大鼠睾丸修复的作用机制研究

Mechanism of Morinda officinalis Polysaccharides in Regulating Testicular Repair in Rats with Varicocele

目的:探讨巴戟天多糖(MOP)对精索静脉曲张型(VC)大鼠睾丸的保护作用。方法:使用左肾静脉缩窄法制备VC模型,将60只大鼠按简单随机法分为空白对照组、模型组、VC组、VC+100 mg/kg巴戟天多糖组、VC+200 mg/kg巴戟天多糖组、VC+300 mg/kg巴戟天多糖组。通过GeneCards数据库筛选VC睾丸修复相关基因;酶联免疫吸附试验法检测促性腺激素释放激素(GnRH)、睾酮(T)、黄体生成素(LH)、卵泡刺激素(FSH)水平;检测左侧睾丸相关指标及活性氧(ROS)含量;TUNEL检测细胞凋亡;蛋白质免疫印迹检测磷酸酯酶与人第10号染色体缺失的磷酸酶及张力蛋白同源的基因蛋白(PTEN)、磷脂酰肌醇3-激酶(PI3K)、蛋白激酶B(AKT)蛋白表达。结果:相较于模型组,VC组睾丸间质面积、细胞凋亡率、ROS、GnRH、LH、FSH及PI3K、AKT、p-AKT蛋白表达显著升高,睾丸与附睾系数、曲精小管直径、附睾精子数、T及PTEN蛋白表达显著降低(P<0.05);相较于VC组,VC+100 mg/kg组睾丸间质面积、细胞凋亡率、ROS、FSH及PI3K、AKT、p-AKT蛋白表达显著降低,睾丸与附睾系数、曲精小管直径、附睾精子数、T及PTEN蛋白表达显著升高(P<0.05);相较于VC组,VC+200 mg/kg组、VC+300 mg/kg组上述指标结果均相反(P<0.05)。结论:MOP可能通过调控PTEN/PI3K/AKT通路来修复VC大鼠睾丸。

Objective:To investigate the protective effects of Morinda officinalis polysaccharides(MOP)on the testes of rats with varicocele(VC).Methods:A VC model was established using the left renal vein constriction method.Sixty rats were randomly divided into a NC group,a model group,a VC group,a VC+100 mg/kg MOP group,a VC+200 mg/kg MOP group,and a VC+300 mg/kg MOP group.Genes related to testicular repair in VC were screened using the GeneCards database.Hormone levels of gonadotropin-releasing hormone(GnRH),testosterone(T),luteinizing hormone(LH),and follicle-stimulating hormone(FSH)were measured using enzyme-linked immunosorbent assay.Indicators related to the left testis and reactive oxygen species(ROS)levels were assessed.TUNEL staining was performed to detect apoptosis,and Western blot was used to measure the expression of phosphatase and tensin homolog deleted on chromosome 10(PTEN),phosphoinositide 3-kinase(PI3K),and protein kinase B(AKT)proteins.Results:Compared with the model group,the VC group exhibited significantly increased testicular interstitial area,apoptosis rate,ROS,GnRH,LH,FSH,and expression levels of PI3K,AKT,and p-AKT,and significantly decreased testicular-epididymal coefficient,seminiferous tubule diameter,epididymal sperm count,T,and PTEN protein expression(P<0.05).In the VC+100 mg/kg MOP group,testicular interstitial area,apoptosis rate,ROS,FSH,and expression levels of PI3K,AKT,and p-AKT were significantly reduced,while the testicular-epididymal coefficient,seminiferous tubule diameter,epididymal sperm count,T,and PTEN expression were significantly increased compared with the results in the VC group(P<0.05).In contrast,the VC+200 mg/kg MOP and VC+300 mg/kg MOP groups showed opposite results(P<0.05).Conclusion:MOP may repair the testes in VC rats by regulating the PTEN/PI3K/AKT signaling pathway.