高原缺氧寒冷应激环境功能性肠病大鼠模型胃肠组织病理学变化

Changes of Gastrointestinal Histopathology in Rat of Functional Bowel Disorder induced by Hypoxic Cold Stress at Simulated High Altitude Environment

目的探讨高原缺氧寒冷应激环境功能性肠病(FBD)大鼠胃肠组织病理学变化特点并探究微生态制剂干预对其胃肠组织的保护效果。方法建立高原缺氧寒冷应激环境FBD大鼠模型,采集平原对照组(PY)、平原模型组(PM)、高原对照组(GD)和高原模型组(GM)及4种微生态制剂干预组大鼠的胃、回肠和结肠组织进行HE染色,光镜下观察各组织结构变化并进行病理学损伤评分。结果与PY组比较,PM组、GD组和GM组大鼠肠黏膜均可见部分黏膜上皮细胞脱落坏死,固有层裸露,黏膜下层及黏膜下间质、黏膜肌层毛细血管扩张充血,少量炎性细胞浸润。与GM组相比,微生态制剂干预组大鼠的病理损伤明显减轻,其中GS、FMT组干预效果优于DR和DRP组。结论高原缺氧寒冷应激环境功能性肠病大鼠胃肠组织发生明显的病理学改变,微生态制剂干预对胃肠组织有一定的保护作用。

Objective To investigate the characteristics of gastrointestinal histopathological changes in rats with Functional Bowel Disorder(FBD)exposed to hypoxia and cold stress at high altitude and the protective effect of microecological agents on the gastrointestinal tissue.Method The rat model of FBD under hypoxic cold stress at high altitude was established,and the gastric,ileum and colon tissues of the rats in the plain control group(PY),plain model group(PM),plateau control group(GD),plateau model group(GM)and four kinds of microecological preparation intervention groups were collected for HE staining.The structural changes of the tissues were observed under light microscope and pathological damage scores were performed.Result Compared with PY group,part of mucosal epithelial cells were exfoliated and necrotic,lamina propria was exposed,submucosal and submucosal interstitium and mucosal myoma were dilated and congested,and a small amount of inflammatory cells were infiltrated in the intestinal mucosa of PM,GD and GM groups.Compared with GM group,the pathological damage of rats in the microecological preparation group was significantly reduced,and the intervention effect of GS and FMT groups was better than that of DR And DRP groups.Conclusion There are obvious pathological changes in the gastrointestinal tissues of rats with Functional Bowel Disorder under hypoxic and cold stress at high altitude,and the intervention of microecological preparations has a certain protective effect on the gastrointestinal tissues.