不同抗凝比例对SD大鼠凝血指标的影响

Effect of Different Anticoagulation Ratio on Blood Coagulation Index in SD Rats

目的探讨不同抗凝比例对SD大鼠凝血结果的影响。方法SD大鼠80只,雌雄各半。腹腔静脉采血,部分进行血钙(Ca^(2+))、血细胞比容(HCT)和血小板(PLT)的检测,其余按照枸橼酸钠抗凝管1∶2、1∶4、1∶6、1∶8和1∶10的抗凝比例,进行凝血酶原时间(PT)和活化部分凝血活酶时间(APTT)的检测,并分析结果。结果SD大鼠的Ca2+和HCT与人类正常值相近,但PLT远大于人类PLT的正常值范围。APTT随着抗凝剂量增加时间延长,各抗凝组间差异有统计学意义(P<0.05),PT随着抗凝剂量增加时间延长,各抗凝组与1∶2或1∶4组间差异有统计学意义(P<0.05)。APTT在抗凝比1∶8~1∶10时凝固曲线基线期缩短,PT在抗凝比1∶2~1∶4时凝固曲线有异常波动。结论SD大鼠最优抗凝比应适当高于人类,在1∶6左右能降低不合格标本的检出率,给动物实验提供更可靠的检测结果。

Objective To explore the effect of different anticoagulant ratio on coagulation result in SD rats.Method There were 80 SD rats,half male and half female.After abdominal venous blood collection.Half of them were tested for blood calcium(Ca^(2+)),hematocrit(HCT)and platelets(PLT),the remaining rats were tested for prothrombin time(PT)and activated partial thromboplastin time(APTT)according to the anticoagulant ratio of 1∶2,1∶4,1∶6,1∶8 and 1∶10.Result The Ca2+and HCT values of SD rats were close to the normal values of human,but PLT was much larger than the normal value range of human.The time of APTT were prolonged with the increase of anticoagulant dose(P<0.05),the time of PT were prolonged with the increase of anticoagulant dose,and the difference between other anticoagulant groups and 1∶2 or 1∶4 groups was statistically significant(P<0.05).The baseline of APTT were shortened when the anticoagulation ratio were 1∶8-1∶10,and the coagulation curve of PT were abnormal when the anticoagulation ratio were 1∶2-1∶4.Conclusion The optimal anticoagulation ratio of SD rats should be appropriately higher than that of human being,and it can reduce the rate of unqualified sample detection at 1∶6.It can provide more reliable detection result for animal experiments.