LC-MS/MS法同时测定连花清瘟胶囊中8种成分

Simultaneous determination of eight constituents in Lianhua Qingwen Capsules by LC-MS/MS

目的建立LC-MS/MS法同时测定连花清瘟胶囊中连翘苷、连翘酯苷A、绿原酸、新绿原酸、苦杏仁苷、大黄素、大黄酸、红景天苷的含量。方法分析采用ACQUITY UPLC HSS T3色谱柱(100 mm×2.1 mm,1.8μm);流动相0.1%甲酸-乙腈,梯度洗脱;体积流量0.3 mL/min;柱温35℃;电喷雾离子源;负离子扫描;多反应监测模式。结果8种成分在各自范围内线性关系良好(r≥0.9995),平均加样回收率99.20%~100.96%,RSD 0.62%~1.23%。结论该方法简便、灵敏、可靠,可用于连花清瘟胶囊的质量控制。

AIM To establish an LC-MS/MS method for the simultaneous content determination of forsythin,forsythoside A,chlorogenic acid,neochlorogenic acid,amygdalin,emodin,rhein and salidroside in Lianhua Qingwen Capsules.METHODS The analysis was performed on a 35℃thermostatic ACQUITY UPlC-HSS T3 column(100 mm×2.1 mm,1.8μm),with the mobile phase comprising of 0.1%formic acid-acetonitrile flowing at 0.3 mL/min in a gradient elution manner,and electron spray ionization source was adopted in negative ion scanning with multiple reaction monitoring mode.RESULTS Eight constituents showed good linear relationships within their own ranges(r≥0.9995),whose average recoveries were 99.20%-100.96%with the RSDs of 0.62%-1.23%.CONCLUSION This simple,sensitive and reliable method can be used for the quality control of Lianhua Qingwen capsules.