基于多肽水凝胶材料的肿瘤类器官培养体系的建立

Development of a tumor organoid culture system with peptide-based hydrogels

多肽水凝胶是一类具有特殊网络结构的高分子材料,因其性质稳定、生物相容性良好等特点被广泛应用于生物医药领域。环境响应型自组装多肽水凝胶在环境改变时发生响应,多肽自组装形成纳米纤维网状结构,更好地模拟了细胞外基质和细胞生长微环境,可用于细胞的3D培养和类器官培养。为了建立CulXⅡ多肽水凝胶材料的肿瘤类器官培养体系,本研究选用了Panc-1、U87、H358细胞,采用半球体的培养方式,通过CulXⅡ多肽水凝胶材料包裹肿瘤细胞在24孔板中培养15 d。胰腺癌肿瘤类器官呈现出3D立体球体的形态,类器官大小随培养时间的延长而增大,最终直径大小为150-300μm。肿瘤类器官数量较多、大小不一、细胞活力较好、边缘轮廓清晰、形态较好,培养比较成功。本研究在CulXⅡ多肽水凝胶材料基础上建立了肿瘤类器官模型,为研究肿瘤发病机制、新药研发、肿瘤抑制提供了研究模型。

Peptide-based hydrogel,the polymer materials with a special network structure,are widely used in various fields of biomedicine due to their stable properties and biocompatibility.Environment-responsive self-assembled peptide aqueous solutions can respond to environment changes by the self-assembly of peptides into nanofiber networks.Peptide-based hydrogels well simulate the extracellular matrix and cell growth microenvironment,being suitable for 3D cell culture and organoid culture.To establish a tumor organoid culture system with peptide-based hydrogels,we cultured Panc-1,U87,and H358 cells in a 3D spherical manner using CulXⅡpeptide-based hydrogels in 24-well plates for 15 days.The organoids showed a 3D spherical shape,and their sizes increased with the extension of the culture time,with a final diameter ranging from 150 to 300μm.The organoids had a large number,varying sizes,good cell viability,clear edges,and a good shape,which indicated successful organoid construction.The tumor organoid culture system established in this study with CulXⅡpeptide-based hydrogels provides a model for studying tumor pathogenesis,drug development,and tumor suppression.