基于铬离子掺杂镓酸锌的近红外化学发光纳米探针制备及其过氧化氢传感研究

Cr^(3+)-Doped zinc galletes-based near-infrared chemiluminescent nanoprobe for H_(2)O_(2)sensing

化学发光法因其灵敏度高、选择性好、响应速度快等优异性能在分析传感领域引起广泛关注.然而目前发展发光性能优异且发射波长长的新型化学发光体系仍面临严峻挑战.本文通过将具有优异反应活性和近红外光学发射信号的铬离子掺杂镓酸锌(Cr^(3+)-doped ZGC)纳米材料引入新型化学发光体的构建中,发展了基于ZGC-Fe^(2+)-H_(2)O_(2)的新型近红外化学发光体系,成功用于H_(2)O_(2)的高效传感研究.更进一步地,通过对ZGC-Fe^(2+)-H_(2)O_(2)体系的化学发光光谱及活性氧自由基清除实验研究,提出了一种可能的基于活性氧注入式的“电子-空穴复合”发光机制.得益于化学发光信号强度高且免背景信号干扰的独特优势,该ZGC-Fe^(2+)-H_(2)O_(2)近红外化学发光法在检测H_(2)O_(2)时具有宽线性范围(10-100μM),高灵敏度、检测限低至4.68μM以及优异的抗干扰性和选择性,为其他化学发光检测平台的构建提供了新思路.

Chemiluminescence analysis gained popularity in optical sensing applications owing to its exceptional qualities of high sensitivity,excellent selectivity,as well as fast response time.Regrettably,it is still quite difficult to develop innovative chemiluminescence systems with extended emission bands and good signal-to-noise ratios.In this study,the Cr^(3+)-doped zinc galletes(ZGC)-ferrous ions(Fe^(2+))-hydrogen peroxide(H_(2)O_(2))-based chemiluminescence system was fabricated by introducing ZGC nanoparticles with great reactivity and near-infrared emission band,which enabled remarkable H_(2)O_(2)analytical performance.Additionally,using chemiluminescence spectroscopy and reactive oxygen species(ROS)related free radical scavenging studies,a new concept of chemiluminescence mechanism—“ROS-triggered electron-hole annihilation”was proposed and thoroughly investigated.Benefitting from its high chemiluminescence signal and autofluorescence-free background,the established ZGC-Fe^(2+)-H_(2)O_(2)system demonstrated wide linear range between 10μM and 100μM,high sensibility with a detection limit(LOD)as low as 4.68μM,and excellent anti-interference ability and selectivity,indicating it potential application on other chemiluminescence system constructions for highly accurate analysis.