Hydration transformation behaviors of CO_(2) and excellent anti-inflammatory activity on RAW 264.7 cell

二氧化碳在水中的相转化及其优异的抗炎生理活性

Skin care products with carbonic acid(H_(2)CO_(3))have gained extensive attention worldwide.However,the conversion of CO_(2) to H_(2)CO_(3) is not stable,and the mechanism of the effect of H_(2)CO_(3) on skin care has not been clearly proved.The hydration-dissolution behaviors of CO_(2) were investigated under different temperature,pH,and pressure conditions.Moreover,based on the phenomenon of CO_(2) hydration transformation,the inflammatory effect of CO_(2) hydrate on macrophages(RAW 264.7)was investigated.The result shows that the increase in temperature weakened the hydration of CO_(2),and the increase in pH and pressure both promoted the water-phase transformation of CO_(2).When pH<6,CO_(2) reacts with water to generate H_(2)CO_(3).When pH was between 6-7,the prompt solution was a mixture of H_(2)CO_(3) and HCO_(3)^(-).When the pH was between 7-9,they mainly generated HCO_(3)^(-).And when pH>9,CO_(2) solubility mainly converts to CO_(3)^(2-).Besides,CO_(2) can inhibit the secretion of inflammatory factors by RAW 264.7 cells by inhibiting the phosphorylation of the p38 protein.CO_(2) hydrate inhibited the expression of pro-inflammatory factors IL-6,TNF-α,and up-regulated the expression of anti-inflammatory factor IL-10.Furthermore,the anti-inflammatory molecular mechanism of CO_(2) hydration inhibited the MAPK signaling pathway by inhibiting the phosphorylation of p38.The hydration-dissolution behavior of CO_(2) was investigated.This work revealed the anti-inflammatory bioeffect of CO_(2) hydrate,providing a theoretical basis and application support for CO_(2) skin care products.

含有碳酸(H_(2)CO_(3))的护肤品在全球范围内受到广泛关注。然而,二氧化碳(CO_(2))转化为H_(2)CO_(3)的过程并不稳定,H_(2)CO_(3)护肤的作用机理也尚未得到明确证实。本文对CO_(2)在不同温度、p H值和压力条件下的水合-溶解行为进行了研究。此外,基于CO_(2)水合转化现象,研究了CO_(2)水合物对巨噬细胞(RAW 264.7)的炎症效应。结果表明,温度升高会减弱CO_(2)的水合作用,而p H值和压力的升高都会促进CO_(2)水相转化。当pH值小于6时,CO_(2)与水反应生成H_(2)CO_(3)。当p H值在6~7之间时,提示溶液是H_(2)CO_(3)和HCO_(3)^(-)的混合物。当p H值在7~9之间时,它们主要生成HCO_(3)^(-)。当p H值大于9时,CO_(2)的溶解度主要转化为CO_(3)^(2-)。此外,CO_(2)还能通过抑制p38蛋白的磷酸化来抑制RAW 264.7细胞分泌炎症因子。CO_(2)水合物抑制了促炎因子IL-6、TNF-α的表达,并上调了抗炎因子IL-10的表达。此外,CO_(2)水合的抗炎分子机制是通过抑制p38的磷酸化来抑制MAPK信号通路。研究了CO_(2)的水合溶解行为,这项研究揭示了CO_(2)水合物的抗炎生物效应,为CO_(2)护肤品提供了理论依据和应用支持。