雪菊与芦荟醇提物对UVB导致的小鼠皮肤光损伤的保护作用

Protective effect of snow chrysanthemum and Aloe vera alcoholic extract on photodamage of skin in mice caused by UVB

雪菊和芦荟在保护皮肤光损伤方面有极大的潜力。文章研究了雪菊和芦荟的乙醇提取物对户外紫外线(Ultraviolet radiation B,UVB)诱导的小鼠皮肤光损伤的保护作用。实验将小鼠分为正常对照组(NC组)、模型对照组(MC组)、雪菊醇提物组(SCAE组)、芦荟醇提物组(AVAE组)、雪菊芦荟醇提物组(SCAE+AVAE组)。通过对受损皮肤中抗氧化指标、羟脯氨酸(Hydroxyproline,HYP)含量和小鼠血清相关炎症因子的测定,初步揭示预防小鼠皮肤光损伤的作用机制。结果表明,与MC组相比,三个干预组对UVB诱导的小鼠皮肤外观均有明显改善,通过苏木精-伊红染色法(Hematoxylin-eosin staining,HE)得出,SCAE+AVAE组和SCAE组比AVAE组效果更好,小鼠皮肤更加健康有弹性。三个干预组也可降低UVB诱导小鼠皮肤的氧化应激损伤,SCAE+AVAE组丙二醛(Malondialdehyde,MDA)含量仅为(28.18±4.25) nmol/mgprot,显著低于其他组;SCAE+AVAE组三种抗氧化酶:过氧化氢酶(Catalase,CAT)、超氧化物歧化酶(Superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-Px)活性也显著升高。SCAE+AVAE组较单独醇提物组效果更佳,HYP含量达到了(4.12±0.18)μg/mg。三个干预组均可下调炎症因子含量,抑制炎症反应,其中SCAE+AVAE组效果更优,涂抹SCAE+AVAE使白细胞介素-1 (Interleukin-1,IL-1)、白细胞介素-6 (Interleukin-6,IL-6)和肿瘤坏死因子α (Tumour necrosis factor-α,TNF-α)的活性分别降低了38.93%,43.31%,46.34%。雪菊和芦荟的协同作用在护肤美容等领域具有一定应用前景。

Snow chrysanthemum and Aloe vera have great potential to protect the skin from photodamage.In this paper,we investigated the protective effects of ethanolic extracts of snow chrysanthemum and Aloe vera against outdoor UVB-induced skin photodamage in mice.According to the experimental requirements,the mice were divided into a normal control group(NC group),a model control group(MC group),a snow chrysanthemum alcoholic extract group(SCAE group),a Aloe vera alcoholic extract group(AVAE group),and a snow chrysanthemum and Aloe vera alcoholic extract group(SCAE+AVAE group).In order to reveal the protective mechanism of alcoholic extract against photodamage in mouse skin,antioxidant indexes,hydroxyproline(HYP)content and serum-associated inflammatory factors in damaged skin were determined in mice.The results show that all three intervention groups significantly improve UVB-induced skin appearance in mice compared to the MC group.By hematoxylin-eosin staining(HE),it indicates that the SCAE+AVAE and SCAE are more effective than the AVAE,and the mice have healthier and more elastic skin.The three intervention groups also reduce UVB-induced oxidative stress damage in mice skin,and the malondialdehyde(MDA)content of the SCAE+AVAE group is only(28.18±4.25)nmol/mgprot,which is significantly lower than that of the other groups.The activities of three antioxidant enzymes:catalase(CAT),superoxide dismutase(SOD),and glutathione peroxidase(GSH-Px)are also significantly higher in the SCAE+AVAE group.The SCAE+AVAE group is more effective than the alcoholic extract alone group,and the HYP content reaches(4.12±0.18)μg/mg.All three intervention groups can down-regulate the content of inflammatory factors and inhibit the inflammatory response,among which the SCAE+AVAE group is more effective.And the application of SCAE+AVAE reduces the activities of interleukin-1(IL-1),interleukin-6(IL-6)and tumour necrosis factor-α(TNF-α)by 38.93%,43.31%and 46.34%,respectively.Notably,the effect of the combined extracts is much greater than that of the extracts singly at the same dose.This study reveals the synergistic protective effect of SCAE+AVAE in the treatment of UV-induced photodamage,which is promising for skin care and cosmetic applications.