新城疫、血清4型和8型禽腺病毒病二联三价嵌合型病毒样颗粒的构建及鉴定

Construction and identification of bivalent chimeric virus-like particles of Newcastle disease virus,serotype 4 and serotype 8 avian adenovirus

基于昆虫杆状病毒表达系统和新城疫病毒样颗粒载体平台,采用间接免疫荧光、Western blot和透射电镜等方式,构建一种表面展示NDV HN蛋白、FAdV4 Fiber-2蛋白、FAdV-8a Fiber蛋白和FAdV-8b Fiber蛋白的二联三价嵌合型病毒样颗粒(ND-FAdV-4/8a/8b cVLPs)。结果显示,成功构建了表达FAdV-8a Fiber和FAdV-8b Fiber蛋白的重组杆状病毒rBV-c8aFiber和rBV-c8bFiber。此外,ND-FAdV-4/8a/8b cVLPs各组分蛋白均正确表达,是一种约100 nm、具备囊膜和纤突的纳米颗粒,为新城疫、禽腺病毒病的防控提供了一种安全的病毒样颗粒新型疫苗候选株。

A bivalent chimeric virus-like particle(ND-FAdV-4/8a/8b cVLPs)displaying NDV HN protein,FAdV4 fiber-2 protein,FAdV-8a Fiber protein and FAdV-8b Fiber protein was constructed based on insect baculovirus expression system and Newcastle disease virus-like particle vector platform and was identified using indirect immunofluorescence,Western blot and transmission electron microscopy.The results showed that recombinant baculoviruses rBV-c8aFiber and rBV-c8bFiber expressing FAdV-8a Fiber and FAdV-8b Fiber were successfully constructed.In addition,all components of ND-FAdV-4/8a/8b cVLPs were correctly expressed,and ND-FAdV-4/8a/8b cVLPs was a nanoparticle about 100 nm in size,with a capsule membrane and fibers,providing a green and safe virus-like particle vaccine candidate for the control of Newcastle disease and avian adenovirus disease.