麻杏石甘汤对咳嗽变异性哮喘大鼠肺组织损伤的影响

Effects of Maxing Shigan decoction on lung tissue damage in cough variant asthma rats

目的探究麻杏石甘汤通过调节Notch1/Jagged1信号通路对咳嗽变异性哮喘(CVA)大鼠肺组织损伤的影响。方法通过卵清蛋白+氢氧化铝致敏法建立CVA大鼠模型,建模成功后将大鼠随机分为对照组(常规饲养)、模型组(4%卵清蛋白0.5 mL+2%氢氧化铝0.2 mL)、联合组(在模型组的基础上分别加16 g·kg^(-1)麻杏石甘汤和200 mg·kg^(-1)Jagged1)和低、中、高剂量实验组(在模型组的基础上分别加4、8和16 g·kg^(-1)麻杏石甘汤),每组15只。观察大鼠的咳嗽次数及肺部组织病理学变化,用蛋白质印迹法检测Notch1、Jagged1蛋白的表达水平。结果对照组、模型组、低剂量实验组、中剂量实验组、高剂量实验组和联合组大鼠的咳嗽次数分别为(2.04±0.08)、(9.21±0.82)、(7.38±0.60)、(5.44±0.42)、(3.14±0.32)和(7.88±0.65)次,肺组织杯状细胞个数分别为(13.65±1.24)、(59.66±5.54)、(47.21±4.12)、(34.58±3.01)、(23.54±2.06)和(39.25±3.68)个,Notch1蛋白相对表达水平分别为0.42±0.03、1.16±0.09、0.97±0.09、0.76±0.07、0.59±0.05和0.89±0.07,Jagged1蛋白相对表达水平分别为0.36±0.03、1.09±0.04、0.92±0.09、0.70±0.07、0.46±0.04和0.79±0.07。模型组的上述指标与对照组和低、中、高剂量实验组比较,联合组的上述指标与高剂量实验组比较,在统计学上差异均有统计学意义(均P<0.05)。结论麻杏石甘汤可能通过抑制Notch1/Jagged1信号通路激活,改善CVA大鼠肺组织损伤。

Objective To investigate the impact of Maxing Shigan decoction on lung tissue damage in cough variant asthma(CVA)rats by regulating the Notch1/Jagged1 signaling pathway.Methods The CVA rat model was established by ovalbumin+aluminum hydroxide sensitization method.After successful modeling,the rats were randomly divided into control group(conventional feeding),model group(4%ovalbumin 0.5 mL+2%aluminum hydroxide 0.2 mL),combined group(on the basis of the model group,16 g·kg^(-1)Maxing Shigan decoction and 200 mg·kg^(-1)Jagged1 were added respectively)and experimental-L,experimental-M,experimental-H groups(on the basis of the model group,4,8 and 16 g·kg^(-1)Maxing Shigan decoction were added respectively),with 15 rats in each group.The cough frequency and histopathological changes of the lung tissue were observed,and the expression level of Notch1 and Jagged1 protein were detected by Western blot.Results The coughing times of rats in control group,model group,experiment-L group,experiment-M group,experiment-H group and combined group were(2.04±0.08),(9.21±0.82),(7.38±0.60),(5.44±0.42),(3.14±0.32)and(7.88±0.65)times,respectively;the number of goblet cells in lung tissue was 13.65±1.24,59.66±5.54,47.21±4.12,34.58±3.01,23.54±2.06 and 39.25±3.68,respectively;the relative expression levels of Notch1 protein were 0.42±0.03,1.16±0.09,0.97±0.09,0.76±0.07,0.59±0.05 and 0.89±0.07,respectively;the relative expression levels of of Jagged1 protein were 0.36±0.03,1.09±0.04,0.92±0.09,0.70±0.07,0.46±0.04 and 0.79±0.07,respectively.The above indexes of the model group were compared with those of the control group,the experimental-L group,the experimental-M group and the experimental-H group,and the above indexes of the combined group were compared with those of the experimental-H group,and the differences were statistically significant(all P<0.05).Conclusion Maxing Shigan decoction may improve lung tissue damage in CVA rats by inhibiting the activation of Notch1/Jagged1 signaling pathway.