姜黄素对人结直肠癌SW-620细胞抗肿瘤效果的研究

Anti-tumor effect of curcumin on human colorectal cancer SW-620 cells

目的:研究姜黄素(Cur)对人结直肠癌SW-620细胞生长、迁移的抑制作用。方法:不同浓度Cur(2、5、10μg/mL)处理SW-620细胞后,采用MTT法检测细胞增殖能力变化,流式细胞仪分别结合Rh123、DCFH-DA、Annexin V-FITC/PI染色检测细胞线粒体膜电位(Δψ_(m))变化、活性氧簇(ROS)生成及细胞凋亡情况,彗星电泳观察细胞DNA损伤情况,荧光显微技术观察细胞凋亡特征,Transwell实验检测细胞迁移能力,扫描电子显微镜观察细胞表面结构变化。结果:与对照组相比,5、10μg/mL Cur处理24、48 h可显著抑制细胞增殖(t=5.28、21.8、18.19、33.19,均P<0.01),半数抑制浓度分别为6.46、3.75μg/mL。药物处理4 h,与对照组相比,2、5、10μg/mL Cur组细胞内ROS生成分别增加至15.8%、30.2%和45%(t=3.64、7.4、13.8,均P<0.05);Δψ_(m)下降的细胞比例分别为22.7%、38.5%和72.7%(t=7.7、11.32、45.26,均P<0.01);药物处理12、24 h,2、5、10μg/mL Cur组凋亡细胞比例分别为6.6%(t=3.79,P=0.11)、32.6%、68.7%(t=21.3、64.39,均P<0.01)和16.9%(t=10.37,P<0.05)、47.2%、78.9%(t=23.46、19.8,均P<0.01);药物处理12 h,2、5、10μg/mL Cur组迁移细胞的数量分别减少至79.66%(t=7.14,P<0.05)、53.36%和39.45%(t=21.91、25.04,均P<0.01)。结论:Cur可抑制人结直肠癌SW-620细胞增殖和迁移。

Objective:To study the inhibitory effect of curcumin(Cur)on the growth and migration of human colorectal cancer SW-620 cells.Methods:After SW-620 cells were treated with different concentrations of Cur(2,5,10μg/mL),MTT assay was used to detect the changes in cell proliferation ability compared with the control group.The changes of mitochondrial membrane potential(Δψ_(m)),generation of reactive oxygen species(ROS)and apoptosis were detected by flow cytometry with Rh123,DCFH-DA and Annexin V-FITC/PI staining,respectively.Comet assay was used to detect DNA damage.Cell apoptosis was observed by fluorescence microscopy.Transwell assay was used to detect cell migration ability,and scanning electron microscopy was used to observe the changes of cell surface structure.Results:Compared with the control group,the proliferation of SW-620 cells was significantly inhibited by 5,10μg/mL Cur for 24 and 48 h(t=5.28,21.8,18.19,33.19,all P<0.01),and the half maximal inhibitory concentrations were 6.46 and 3.75μg/mL,respectively.2,5,10μg/mL Cur group induced intracellular ROS production increased to 15.8%,30.2%and 45%after 4 h of Cur treatment(t=3.64,7.4,13.8,all P<0.05),and the proportion of cells withΔψ_(m)decreased to 22.7%,38.5%and 72.7%(t=7.7,11.32,45.26,all P<0.01),respectively.The proportions of apoptotic cells after 12 and 24 h treatment in 2,5,10μg/mL Cur group were 6.6%(t=3.79,P=0.11),32.6%,68.7%(t=21.3,64.39,both P<0.01)and 16.9%(t=10.37,P<0.05),47.2%,78.9%(t=23.46,19.8,both P<0.01).The cell migration rate in 2,5,10μg/mL Cur group were decreased to 79.66%(t=7.14,P<0.05),53.36%and 39.45%at 12 h,respectively(t=21.91,25.04,both P<0.05).Conclusion:Cur can inhibit the proliferation and migration of human colorectal cancer SW-620 cells.