LncRNA FAM83H-AS1和miR-136在上皮性卵巢癌中的表达及临床意义

Expression and clinical significance of LncRNA FAM83H-AS1 and miR-136 in epithelial ovarian cancer

目的:探讨长链非编码RNA(LncRNA)FAM83H-AS1和miR-136在上皮性卵巢癌(EOC)组织中的表达及其临床意义。方法:收集2019年6月—2021年6月在郑州人民医院妇科手术治疗的68例EOC患者的EOC组织(EOC组)及68例非肿瘤卵巢组织(对照组),检测两组FAM83H-AS1及miR-136的表达水平并分析二者的相关性;根据FAM83H-AS1及miR-136表达的中位值将EOC患者分为FAM83H-AS1低表达组、高表达组和miR-136低表达组、高表达组,并分析不同分组EOC患者临床病理特征、无进展生存期(PFS)和总生存期(OS)的差异;将miR-136 mimic及mimic-NC分别与FAM83H-AS1-wt、FAM83H-AS1-mut共转染至293T细胞中,然后采用双荧光素酶报告基因实验证实FAM83H-AS1与miR-136的靶向关系。结果:与对照组相比,EOC组FAM83H-AS1表达水平明显升高,miR-136表达水平明显降低(t=21.636、22.050,均P<0.05),且二者呈负相关(r=-0.283,P=0.019)。不同FAM83H-AS1、miR-136分组间FIGO分期(χ^(2)=13.247、4.769,均P<0.05)及淋巴结转移均有明显差异(χ^(2)=11.698、4.140,均P<0.05)。FAM83H-AS1高表达组患者的OS和PFS低于低表达组(均P<0.05),而miR-136高表达组患者的OS和PFS高于低表达组(均P<0.05);FAM83H-AS1是EOC患者PFS的独立影响因素之一(HR=2.438,95%CI:1.055~5.638,P<0.05),而miR-136是OS的独立影响因素之一(HR=0.401,95%CI:0.162~0.991,P<0.05)。双荧光素酶报告基因实验证实FAM83H-AS1与miR-136存在靶向关系。结论:FAM83H-AS1与miR-136在EOC中异常表达,二者均可作为EOC患者的潜在预后评估指标。

Objective:To investigate the expression and clinical significance of lncRNA FAM83H-AS1 and miR-136 in epithelial ovarian cancer(EOC)tissues.Methods:A total of 68 cases of EOC tissue(EOC group)and 68 cases of non-tumor ovarian tissue(control group)were collected in the Department of Gynecology,Zhengzhou People′s Hospital from June 2019 to June 2021,the expression levels of FAM83H-AS1 and miR-136 in two groups were detected and the correlation between two groups was analyzed.According to the median value of FAM83H-AS1 and miR-136 expression,patients with EOC were divided into FAM83H-AS1 low expression group,high expression group,and miR-136 low expression group and high expression group.The clinicopathological characteristics,progression-free survival(PFS)and overall survival(OS)of EOC patients in different groups were analyzed.MiR-136 mimic and mimic-NC were co-transfected into 293T cells with FAM83H-AS1-wt and FAM83H-AS1-mut,respectively,and then dual luciferase reporter gene assay was used to verify the targeting relationship between FAM83H-AS1 and miR-136.Results:Compared with the control group,the expression level of FAM83H-AS1 in the EOC group was significantly increased,and the expression level of miR-136 was significantly decreased(t=21.636,22.050,all P<0.05),their expression was reversely correlated(t=-0.283,P=0.019).There were significant differences in FIGO stage(χ^(2)=13.247,4.769,all P<0.05)and lymph node metastasis(χ^(2)=11.698,4.140,all P<0.05)among FAM83H-AS1 and miR-136 groups.The OS and PFS of the high FAM83H-AS1 group were lower than those of the low FAM83H-AS1 group(P<0.05),while the OS and PFS of the high miR-136 group were higher than those of the low miR-136 group(P<0.05).FAM83H-AS1 was an independent influencing factor for PFS in EOC patients(HR=2.438,95%CI:1.055-5.638,P<0.05),while miR-136 was an independent influencing factor for OS in EOC patients(HR=0.401,95%CI:0.162-0.991,P<0.05).The dual luciferase reporter gene assay confirmed the targeting relationship between FAM83H-AS1 and miR-136.Conclusion:FAM83H-AS1 and miR-136 are abnormally expressed in EOC,and both can be used as potential indicators for evaluating the prognosis of EOC patients.