类风湿关节炎患者血清miR-140-5p、VEGF水平与动脉粥样硬化的相关性分析

Correlation between serum miR-140-5p,VEGF levels and atherosclerosis in patients with rheumatoid arthritis

目的分析类风湿关节炎患者血清微小RNA-140-5p(miR-140-5p)、血管内皮生长因子(VEGF)水平与动脉粥样硬化的相关性。方法选取2022年1月至2023年9月该院收治的类风湿关节炎患者80例为研究组,根据颈动脉超声是否存在中层厚度增厚将研究组分为合并组(46例)与未合并组(34例)。另选取同期在该院体检中心体检的健康者40例为对照组。实时荧光定量PCR(RT-qPCR)和酶联免疫吸附试验(ELISA)检测受试者血清miR-140-5p、VEGF水平,超声检查合并组与未合并组的颈动脉内膜中层厚度。Pearson和Spearman分析类风湿关节炎患者临床资料之间的相关性,并以Logistic回归模型分析影响类风湿关节炎患者并发动脉粥样硬化的因素。结果3组受试者的类风湿因子、红细胞沉降率(ESR)、颈动脉内膜中层厚度、空腹血糖(FPG)、空腹胰岛素(FINS)、胰岛素抵抗指数(HOMA-IR)、超敏C反应蛋白(hs-CRP)、miR-140-5p、VEGF差异有统计学意义(P<0.05),未合并组和合并组的类风湿因子、ESR、颈动脉内膜中层厚度、FPG、hs-CRP、VEGF高于对照组,FINS、HOMA-IR、miR-140-5p低于对照组,差异有统计学意义(P<0.05)。合并组的类风湿因子、颈动脉内膜中层厚度、FPG、hs-CRP、VEGF高于未合并组,差异有统计学意义(P<0.05),FINS、miR-140-5p低于未合并组,差异有统计学意义(P<0.05)。类风湿关节炎患者的类风湿因子、颈动脉内膜中层厚度、hs-CRP与miR-140-5p呈负相关(P<0.05),与VEGF呈正相关(P<0.05),miR-140-5p与VEGF呈负相关(P<0.05)。类风湿因子、颈动脉内膜中层厚度、hs-CRP、VEGF为影响类风湿关节炎患者并发动脉粥样硬化的危险因素,miR-140-5p是保护因素。结论类风湿关节炎患者血清miR-140-5p、VEGF与动脉粥样硬化的发生相关,miR-140-5p是并发动脉粥样硬化的保护因素,VEGF是危险因素。

Objective To analyze the correlation between serum miR-140-5p,vascular endothelial growth factor(VEGF)levels and atherosclerosis in patients with rheumatoid arthritis.Methods A total of 80 patients with rheumatoid arthritis admitted to the hospital from January 2022 to September 2023 were regarded as the study group.The study group was separated into the combined group(46 cases)and the non combined group(34 cases)according to whether there was the middle layer thickening and atheromatous plaque in carotid artery ultrasound.Another 40 healthy individuals who came to physical examination center in the hospital for physical examination were selected as the control group.Quantitative real-time PCR(RT-qPCR)and enzyme linked immunosorbent assay(ELISA)were applied to detect miR-140-5p and VEGF levels in the serum of subjects.Ultrasound was applied to examine the carotid intima-media thickness in the combined group and the non combined group.Pearson and Spearman were applied to analyze the correlation between clinical data of patients with rheumatoid arthritis,and Logistic regression model was applied to analyze the factors influencing atherosclerosis in rheumatoid arthritis patients.Results There were differences in rheumatoid factor,erythrocyte sedimentation rate(ESR),carotid intima-media thickness,fasting blood glucose(FPG),fasting insulin(FINS),insulin resistance index(HOMA-IR),hypersensitive C-reactive protein(hs-CRP),miR-140-5p,and VEGF among the three groups(P<0.05).The rheumatoid factor,ESR,carotid intima-media thickness,FPG,hs-CRP,and VEGF in the non combined group and combined group were higher than those in the control group,while FINS,HOMA-IR,and miR-140-5p were lower than those in the control group,and the differences were statistically significant(P<0.05).The rheumatoid factor,carotid intima-media thickness,FPG,hs-CRP,VEGF in the combined group were higher than those in the non combined group,and the differences were statistically significant(P<0.05),while FINS and miR-140-5p were lower than those in the non combined group,and the differences were statistically significant(P<0.05).The rheumatoid factor,carotid intima-media thickness and hs-CRP in patients with rheumatoid arthritis were negatively correlated with miR-140-5p(P<0.05)and were positively correlated with VEGF(P<0.05),and miR-140-5p was negatively correlated with VEGF(P<0.05).Rheumatoid factor,carotid intima-media thickness,hs-CRP,VEGF were risk factors for atherosclerosis in patients with rheumatoid arthritis,and miR-140-5p was a protective factor(P<0.05).Conclusion Serum miR-140-5p and VEGF in patients with rheumatoid arthritis are associated with atherosclerosis,miR-140-5p is a protective factor for atherosclerosis,and VEGF is a risk factor.