TNFAIP3和LINC00887在透明细胞肾细胞癌中的表达及临床预后意义

Expression and clinical prognostic significance of TNFAIP3 and LINC00887 in clear cell renal cell carcinoma

目的检测肿瘤坏死因子α诱导蛋白3(TNFAIP3)和LINC00887在透明细胞肾细胞癌(ccRCC)组织中的表达水平,并研究其表达水平与临床病理参数和预后的关系。方法选取2013年1月至2018年10月该院收治的101例ccRCC患者,检测TNFAIP3和LINC00887分别在ccRCC癌组织和配对的癌旁组织中的表达水平,分析TNFAIP3、LINC00887表达水平与ccRCC患者临床病理参数和预后关系,并分析ccRCC患者预后不良的影响因素。采用Spearman相关系数分析TNFAIP3、LINC00887表达水平的相关性。结果TNFAIP3在ccRCC中的阳性率(37.62%)显著低于癌旁组织(52.48%),差异有统计学意义(χ^(2)=4.500,P=0.034)。LINC00887在ccRCC中表达水平(1.38±0.61)显著高于在癌旁组织中的表达水平(1.03±0.43),差异有统计学意义(t=5.396,P<0.001)。TNFAIP3蛋白阳性率在肿瘤最大径≥4.5 cm、TNM分期Ⅲ~Ⅳ期患者中低于在肿瘤最大径<4.5 cm、TNM分期Ⅰ~Ⅱ期患者,差异有统计学意义(P<0.05)。LINC00887在肿瘤最大径≥4.5 cm、病理分级Ⅲ~Ⅳ级、TNM分期Ⅲ~Ⅳ期患者中表达高于肿瘤最大径<4.5 cm、病理分级Ⅰ~Ⅱ级、TNM分期Ⅰ~Ⅱ期患者,差异有统计学意义(P<0.05)。与TNFAIP3高表达组相比,TNFAIP3低表达组ccRCC患者预后较差,差异有统计学意义(χ^(2)=5.118,P=0.024);与LINC00887低表达组相比,LINC00887高表达组ccRCC患者预后较差,差异有统计学意义(χ^(2)=4.638,P=0.031)。TNFAIP3低表达,LINC00887高表达,病理分级Ⅲ~Ⅳ级和TNM分期Ⅲ~Ⅳ期是ccRCC患者预后不良的危险因素(P<0.05)。Spearman秩相关分析结果,TNFAIP3、LINC00887表达水平在ccRCC中呈负相关(r=-0.638,P=0.012)。结论ccRCC癌组织中TNFAIP3表达水平下调、LINC00887表达水平上调,且呈负相关性,二者可能共同调控ccRCC发生发展,具有成为评估ccRCC患者预后肿瘤标志物的潜力。

Objective To detect the expression levels of tumor necrosis factor alpha induced protein 3(TNFAIP3)and LINC00887 in clear cell renal cell carcinoma(ccRCC)tissue,and to study their relationship with clinical pathological parameters and prognosis.Methods A total of 101 ccRCC patients admitted to the hospital from January 2013 to October 2018 were selected.The expression levels of TNFAIP3 and LINC00887 were detected in ccRCC cancer tissue and paired adjacent tissues,respectively.The relationship between TNFAIP3 and LINC00887 expression and clinical pathological parameters and prognosis of ccRCC patients was analyzed,and the influencing factors of poor prognosis in ccRCC patients were also analyzed.Spearman correlation coefficient was used to analyze the correlation between TNFAIP3 and LINC00887 expression.Results The positive rate of TNFAIP3 expression in ccRCC(37.62%)was significantly lower than that in adjacent tissues(52.48%),and the difference was statistically significant(χ^(2)=4.500,P=0.034).The expression level of LINC00887 in ccRCC(1.38±0.61)was significantly higher than that in adjacent tissues(1.03±0.43),and the difference was statistically significant(t=5.396,P<0.001).The positive rates of TNFAIP3 protein in patients with maximum tumor diameter≥4.5 cm and TNM stageⅢ-Ⅳwere lower than those in patients with maximum tumor diameter<4.5 cm and TNM stageⅠ-Ⅱ,and the differences were statistically significant(P<0.05).The positive rates of LINC00887 in patients with maximum tumor diameter≥4.5 cm,pathological gradingⅢ-Ⅳ,and TNM stageⅢ-Ⅳwere higher than those in patients with maximum tumor diameter<4.5 cm,pathological gradingⅠ-Ⅱ,and TNM stageⅠ-Ⅱ,and the differences were statistically significant(P<0.05).Compared with the TNFAIP3 high expression group,the TNFAIP3 low expression group had a poorer prognosis,and the difference was statistically significant(χ^(2)=5.118,P=0.024).Compared with the LINC00887 low expression group of,the LINC00887 high expression group had a poorer prognosis,and the difference was statistically significant(χ^(2)=4.638,P=0.031).Low expression of TNFAIP3,high expression of LINC00887,pathological gradeⅢ-Ⅳ,and TNM stageⅢ-Ⅳwere risk factors for poor prognosis in ccRCC patients(P<0.05).Spearman rank correlation analysis showed that there was a negative correlation between TNFAIP3 and LINC00887 expression in ccRCC tissue(r=-0.638,P=0.012).Conclusion TNFAIP3 expression is down-regulated and LINC00887 expression is up-regulated in ccRCC tissue,and there is a negative correlation.They may jointly regulate the occurrence and development of ccRCC,and have the potential to become tumor markers for evaluating the prognosis of ccRCC patients.