久坐行为与肠易激综合征的因果关系及失眠的介导作用:孟德尔随机化研究

Causal relationship between sedentary behavior and irritable bowel syndrome and the mediating effect of insomnia: Mendelian randomization study

目的通过两样本孟德尔随机化(MR)法探究久坐行为和肠易激综合征(IBS)之间的因果关系,并且量化失眠作为潜在中介因素的作用。方法利用久坐行为的三个表型,即看电视时间、用电脑时间和驾驶时间与IBS的全基因组数据进行两样本MR分析,通过逆方差加权法(IVW)、加权中位数法(WM)、MR-Egger等方法分析暴露与结局之间的风险。然后通过多变量孟德尔随机化(MVMR)量化失眠介导的看电视时间对IBS的影响比例,探究失眠对其的介导作用。结果看电视时间的增加可能会增加IBS的风险,但用电脑时间和驾驶时间与IBS无明显关联。校正失眠后,看电视时间与IBS存在正相关的因果关系;校正看电视时间后,失眠与IBS存在正相关的因果关系,并且失眠的介导比例为21%。结论看电视时间与IBS的发病风险呈正相关,并且可以部分通过失眠来介导,但并未发现用电脑时间和驾驶时间与IBS发生风险相关。

Objective To explore the causal relationship between sedentary behavior and irritable bowel syndrome(IBS)by two-sample Mendelian randomization(MR)method and quantify the role of insomnia as potential mediator.Methods Two-sample MR analysis was performed using the genome-wide data of IBS and three phenotypes of sedentary behavior,namely,watching TV time,using computer time and driving time.The risk between exposure and outcome was analyzed by inverse variance weighting(IVW),weighted median(WM),MR-Egger and other methods.Then,multivariate Mendelian randomization(MVMR)was used to quantify the proportion of insomnia-mediated watching TV time on IBS,and to explore the mediating effect of insomnia on it.Results Increased watching TV time may increase the risk of IBS,but using computer time and driving time are not significantly associated with IBS.After adjusting for insomnia,there was a positive correlation between watching TV time and IBS.After adjusting for watching TV time,there was a positive correlation between insomnia and IBS,and the proportion of insomnia was 21%.Conclusion The watching TV time is positively correlated with the risk of IBS occurrence,and can be partially mediated by insomnia,but no correlation is found between the using computer time and driving time and the risk of IBS occurrence.