五苓散对腹泻型肠易激综合征小鼠体质量、血常规及脏器的影响

Effect of Wuling powder on body weight,routine blood test results,and visceral organs in mice with diarrhea-predominant irritable bowel syndrome

目的:观察五苓散对腹泻型肠易激综合征(IBS-D)小鼠体质量、血常规及脏器的影响。方法:将18只SPF级昆明种小鼠适应性饲养3 d后,随机抽取6只为正常组,其余12只采用番泻叶灌胃结合急性束缚应激的方法制备IBS-D模型,造模成功后将12只IBS-D模型小鼠随机分为自然恢复组和五苓散组,每组各6只。五苓散组以7.93 g/(kg·d)五苓散水煎液进行干预,正常组及自然恢复组小鼠灌胃等频率等剂量蒸馏水。在造模第1天、造模结束日及干预结束日测量小鼠体质量。干预结束后,检测各组小鼠血常规,并计算脏器(肝脏、脾脏及胸腺)指数。结果:造模结束后,与同期正常组相比,模型小鼠体质量显著下降(P<0.05);干预后,五苓散组小鼠的增长速度快于自然恢复组(P<0.05)。与正常组比较,自然恢复组红细胞分布宽度标准差(RDW-SD)、白细胞(WBC)、中性粒细胞计数(NEUT)及五苓散组红细胞(RBC)、RDW-SD、红细胞分布宽度变异系数(RDW-CV)均升高(均P<0.05);五苓散组WBC、NEUT低于自然恢复组,差异有统计学意义(均P<0.05)。结论:五苓散可促进IBS-D小鼠体质量的增长及红细胞的再生,恢复白细胞及中性粒细胞值,有效减轻炎症反应,其对脏器发育及免疫功能的影响还需进一步研究。

Objective:To investigate the effect of Wuling powder on the body weight,routine blood test results,and visceral organs of mice with diarrhea-predominant irritable bowel syndrome(IBS-D).Methods:A total of 18 specific pathogen-free Kunming mice were selected,and after 3 days of adaptive feeding,6 mice were randomly selected as normal group,while the remaining 12 mice were given senna by gavage combined with acute restraint stress to establish a model of IBS-D.After successful modeling,the 12 model mice of IBS-D were randomly divided into spontaneous recovery group and Wuling powder group,with 6 mice in each group.The mice in the Wuling powder group were given the water decoction of Wuling powder at a dose of 7.93 g/(kg·d),and those in the normal group and the spontaneous recovery group were given distilled water by gavage at the same frequency and dose.The body weight of mice was meas-ured on day 1 of modeling and at the end of modeling and intervention.After the end of intervention,routine blood test were measured,and liver index,spleen index,and thymus index were calculated.Results:Compared with the normal group after modeling,the model mice showed a significant reduction in body weight(P<0.05),and after intervention,the Wuling powder group showed a significantly higher growth rate than the spontaneous recovery group(P<0.05).Compared with the normal group,the spontaneous recovery group had significant increases in red blood cell distribution width-standard deviation(RDW-SD),white blood cell count(WBC),and neutrophil count(NEUT),and the Wuling powder group had significant increases in red blood cell count,RDW-SD and red blood cell distribution width-CV(P<0.05).The Wuling powder group had significantly lower WBC and NEUT than the spontaneous recovery group(P<0.05).Conclusion:For mice with IBS-D,Wuling powder can promote the growth of body weight and the regeneration of red blood cells,restore white blood cells and neutrophils,and effectively alleviate inflammatory response.Further studies are needed to observe its effect on visceral organ development and immune function.