基于在体单向肠灌流法探究滑膜炎颗粒的肠吸收特性

Investigation of intestinal absorption characteristics of Huamoyan Granules by in situ single-pass intestinal perfusion method

目的 研究滑膜炎颗粒中丹参素、新绿原酸、原儿茶醛、绿原酸、隐绿原酸、咖啡酸、迷迭香酸和丹酚酸B在小肠不同部位(十二指肠、空肠、回肠)的吸收差异,探究滑膜炎颗粒主要成分的肠吸收特性。方法 建立在体单向肠灌流模型,采用高效液相色谱(HPLC)法检测以上8个指标性成分的浓度;考察不同肠段对8个指标性成分的吸收情况,计算肠吸收参数。结果 8个指标性成分的表观渗透系数(P_(app))均大于1.2×10^(-3) cm·min^(-1),说明各成分在不同肠段中的吸收情况均良好;各成分在空肠的吸收情况最好,其次是回肠和十二指肠。结论 滑膜炎颗粒各指标性成分在小肠中的吸收情况良好,符合口服制剂的要求,为探讨滑膜炎制剂的主要药效成分及其作用特点的研究提供参考。

Objective To study the absorption differences of danshensu,neochlorogenic acid,protocatechualdehyde,chlorogenic acid,cryptochlorogenic acid,caffeic acid,rosmarinic acid,and salvianolic acid B in Huamoyan Granules in different parts of the small intestine(duodenum,jejunum and ileum),and to investigate the intestinal absorption characteristics of the main components of Huamoyan granules.Methods An in situ single-pass intestinal perfusion method was established,and the concentrations of the above eight index components were detected by high performance liquid chromatography(HPLC);the absorption of the eight index components in different intestinal segments was investigated,and the intestinal absorption parameters were calculated.Results The apparent permeability coefficients(P_(app))of the eight index ingredients were all greater than 1.2×10^(-3) cm·min^(-1),indicating that the absorption of each ingredient was good in different intestinal segments;the absorption of each ingredient was best in the jejunum,followed by the ileum and duodenum.Conclusion The absorption of each index ingredient in Huamoyan preparation in the small intestine was good,which was in line with the requirements of oral preparation,and also provided an objective basis for exploring the study of the main pharmacodynamic components of Huamoyan preparation and their action characteristics.