不同组织学染色方法在聚乳酸面部填充剂安全性和有效性临床前动物试验中的应用研究

Application of Different Histological Staining Methods in Preclinical Animal Trials of Polylactic Acid Facial Fillers

目的:探讨HE染色、Masson染色、天狼猩红染色及免疫荧光染色4种不同组织学染色方法在聚乳酸面部填充剂类产品促进胶原再生动物试验研究中的应用价值。方法:选取动物试验研究获得的包埋有聚乳酸面部填充剂的组织标本为研究对象,分别进行组织学染色,显微镜下观察样品周边组织反应情况及I型、III型胶原的再生情况,比较不同种类染色方法在评价动物试验研究胶原再生中的适用性。结果:HE染色可用于浸润炎性细胞类型的鉴定、纤维化及囊腔形成、新生血管形成、脂肪浸润等评价;Masson染色可用于胶原纤维沉积及分布的研究,但无法对胶原进行分型;天狼猩红染色可用于研究胶原新生情况,同时能够在偏振光显微镜下区分I型和III型胶原纤维;免疫荧光染色可特异性的分别标记显现出I型胶原和III型胶原,可用于对胶原纤维的分型。结论:结合多种不同染色方法可实现对组织内炎性细胞类型及数量、胶原沉积、新生血管形成、脂肪浸润、纤维化等组织形态学变化的研究,可用于医美类面部填充剂安全性和有效性动物试验研究。

Objective:To explore the application value of HE staining,Masson staining,Sirius red staining and immunofluorescence staining in the animal experimental study of polylactic acid facial fillers in promoting collagen regeneration.Methods:Tissue specimens embedded with polylactic acid facial fillers obtained from animal experiments were selected for histological staining,and the reaction of surrounding tissues and the regeneration of type I and III collagen were observed under microscope.The applicability of different staining methods in the evaluation of collagen regeneration in animal experiments was compared.Results:HE staining can be used for the identification of infiltrating inflammatory cells,fibrosis and cystic formation,neovascularization and fat infiltration.Masson staining can be used to study the deposition and distribution of collagen fibers,but it can not be used to classify collagen.Sirius red staining can be used to study collagen neovascularization and distinguish type I and type III collagen fibers under polarized light microscope.Immunofluorescence staining can be specifically labeled to show type I collagen and type III collagen respectively,which can be used for the classification of collagen fibers.Conclusions:Combined with different staining methods,the type and number of inflammatory cells,collagen deposition,neovascularization,fat infiltration,fibrosis and other histomorphological changes can be studied.it can be used in animal experimental study on the safety and efficacy of medical facial fillers.