健脾利湿通脉方对深静脉血栓形成大鼠miR-23a及炎性因子影响的研究

The Effects of Jianpi-Lishi-Tongmai Decoction on miR-23a and Inflammatory Factors in Rats with Deep Vein Thrombosis

目的:通过实验观察健脾利湿通脉方对DVT大鼠mi R-23a及相关炎性因子的影响。方法:将140只Wistar大鼠随机分为Blank group、Sham control、Modelset、Low dose group、Middle dose group、High-dose group和Positive control,每组10只,构建DVT模型。分别于给药后7天、14天测定mi R-23a、IL-1β、IL-6及TNF-α含量。结果:1.14 d,与Blank group比较,Modelset大鼠TNF-α、IL-1β、IL-6、及mi R-23a表达显著升高(P<0.05);与Modelset比较,Low dose group、Middle dose group、High-dose group及Positive control大鼠上述指标均显著改善(P<0.05);在TNF-α组别内,与Positive control比较,Blank group、Sham control、Modelset、Low dose group、High-dose group均有显著性差异(P<0.05);在IL-1β组别内,与Positive control比较,Sham control、Modelset、High-dose group均有显著性差异(P<0.05);在IL-6组别内,与Positive control比较,Blank group、Sham control、Middle dose group、High-dose group均有显著性差异(P<0.05);在mi R-23a组别内,与Positive control比较,Blank group、Sham control、Modelset、Low dose group、Middle dose group均有显著性差异(P<0.05)。2.7 d与14 d相比,在大鼠IL-1β、IL-6、TNF-α及mi R-23a四个组别中,Blank group、Sham control前后无明显统计学差异(P>0.05);而Modelset、Low dose group、Middle dose group、High dose group、Positive control前后有明显统计学差异(P<0.05)。结论:健脾利湿通脉方通过下调mi R-23a及IL-1β、IL-6、TNF-α表达而发挥治疗作用。

Objective:To experimentally observe the effects of Strengthening the Spleen,Promoting Dampness and Channeling the Veins Formula on mi R-23a and related inflammatory factors in DVT rats.Methods:140 Wistar rats were randomly divided into Blank group,Sham control,Modelset,Low dose group,Middle dose group,High-dose group and Positive control,10 rats in each group,and DVT model was constructed.The contents of mi R-23a,IL-1β,IL-6 and TNF-αwere measured at 7 and 14 days after drug administration,respectively.Results:1.At 14 d,the expression of TNF-α,IL-1β,IL-6,and mi R-23a was significantly elevated in Modelset rats compared with that in Blank group(P<0.05);the expression of the above indexes was significantly elevated in Low dose group,Middle dose group,High-dose group,and Positive control rats compared with that in Modelset.Positive control rats showed significant improvement in the above indexes(P<0.05);within the TNF-αgroup,Blank group,Sham control,Modelset,Low dose group,High-dose group showed significant differences compared with Positive control(P<0.05);within the IL-1βgroup,there was a significant difference between Sham control,Modelset,and High-dose group when compared with Positive control(P<0.05);within the IL-6 group,Blank group when compared with Positive control,Sham control,Middle dose group,and High-dose group were all significantly different(P<0.05);within the mi R-23a group,compared with Positive control,Blank group,Sham control,Modelset,Low dose group,and Middle dose group were significantly different(P<0.05).2.In the four groups of IL-1β,IL-6,TNF-α,and mi R-23a in rats at 7 d compared with 14 d,there were no statistically significant differences before and after the Blank group,and the Sham control(P>0.05);and the Modelset,Low dose group,Middle dose group,High dose group,Positive control before and after had significant statistical differences(P<0.05).Conclusion:Strengthening the spleen,inducing dampness and promoting circulation formula exerted therapeutic effects by down-regulating mi R-23a and the expression of IL-1β,IL-6,and TNF-α.