干燥综合征患者血清HMGB1、Progranulin与NK细胞亚群和间质性肺疾病的关系研究

Study on the Relationship between Serum HMGB1,Progranulin and NK Cell Subsets and Interstitial Lung Disease in Patients with Sjogren's Syndrome

目的:探讨干燥综合征(SS)患者血清高迁移率族蛋白1(HMGB1)、颗粒蛋白前体(PGRN)与自然杀伤(NK)细胞亚群和间质性肺疾病(ILD)的关系。方法:选择淄博一四八医院2018年9月-2023年12月就诊的89例SS患者作为观察组,另选择同期80例健康体检者作为对照组。Pearson相关性分析SS患者血清HMGB1、PGRN与NK细胞亚群的相关性,多因素Logistic回归分析SS患者发生ILD危险因素。结果:SS患者血清HMGB1、PGRN与CD3^(-)CD16^(+)CD56^(+)NK计数呈负相关(P<0.001)。ILD组HMGB1、PGRN水平高于非ILD组,CD3^(-)CD16^(+)CD56^(+)NK计数低于非ILD组(P<0.05)。多因素Logistic分析显示,高龄、高HMGB1、高PGRN为SS患者发生ILD的危险因素,CD3^(-)CD16^(+)CD56^(+)NK升高为保护因素(均P<0.05)。结论:SS患者中血清HMGB1、PGRN升高,与NK细胞亚群呈负相关。血清HMGB1、PGRN升高、NK细胞亚群降低是SS患者发生ILD的影响因素。

Objective:To investigate the relationship between serum high mobility group protein 1(HMGB1),progranulin(PGRN)and natural killer(NK)cell subsets and interstitial lung disease(ILD)in patients with Sjogren's syndrome(SS).Methods:89patients with SS admitted to the Zibo 148 Hospital from September 2018 to December 2023 were selected as observation group,and 80healthy individuals in same period were selected as control group.The correlation between serum HMGB1,PGRN and NK cell subsets in SS patients were analyzed by Pearson correlation analysis.The risk factors of ILD in SS patients were analyzed by multivariate Logistic regression analysis.Results:Serum HMGB1 and PGRN were negatively correlated with CD3^(-)CD16^(+)CD56^(+)NK count in SS patients(P<0.001).The levels of HMGB1 and PGRN in ILD group were higher than those in non-ILD group,and the CD3^(-)CD16^(+)CD56^(+)NK count was lower than that in non-ILD group(P<0.05).Multivariate Logistic analysis showed that,advanced age,high HMGB1,and high PGRN were risk factors for ILD in SS patients,and elevated CD3^(-)CD16^(+)CD56^(+)NK was a protective factor(all P<0.05).Conclusion:Serum HMGB1 and PGRN are increased in SS patients,which are negatively correlated with NK cell subsets.Elevated serum HMGB1,PGRN and decreased NK cell subsets are the influencing factors of ILD in SS patients.