甲状腺癌组织miR-338-3p、RAB23表达与临床病理特征及预后的关系

Relationship between the Expression of miR-338-3p and RAB23 in Thyroid Cancer Tissues and Clinicopathological Features and Prognosis

目的:探讨甲状腺癌(TC)组织微小核糖核酸-338-3p(miR-338-3p)、Ras癌基因家族成员23(RAB23)表达与临床病理特征及预后的关系。方法:收集217例TC患者术中切除的TC组织、癌旁组织。检测TC组织和癌旁组织中miR-338-3和RAB23 mRNA表达。分析miR-338-3p和RAB23 mRNA表达与TC临床病理特征的关系。Kaplan-Meier法对高/低miR-338-3p、RAB23 mRNA表达组TC患者进行生存分析。多因素Cox回归分析TC患者预后不良的影响因素。结果:与癌旁组织比较,TC组织中miR-338-3p表达降低,RAB23 mRNA表达升高(P<0.05)。miR-338-3p、RAB23 mRNA表达与病理类型、TNM分期、淋巴结转移有关(P<0.05)。随访3年,217例TC患者3年总生存率为83.41%(181/217)。miR-338-3p高表达组3年总生存率高于miR-338-3p低表达组,RAB23 mRNA高表达组3年总生存率低于RAB23 mRNA低表达组(P<0.05)。多因素Cox回归分析结果显示,miR-338-3p<0.62、RAB23 mRNA≥2.13、淋巴结转移、TNM分期Ⅲ~Ⅳ期、甲状腺低/未分化癌为TC患者预后不良的独立危险因素。结论:TC组织中miR-338-3p呈低表达、RAB23 mRNA呈高表达,是TC患者预后不良的危险因素,二者与TC患者病理类型、TNM分期、淋巴结转移有关。

Objective:To investigate the relationship between the expression of microribonucleic acid-338-3p(miR-338-3p)and Ras oncogene family member 23(RAB23)in thyroid cancer(TC)tissues and clinicopathological features and prognosis.Methods:The intraoperatively resected TC tissues and adjacent tissues from 217 TC patients were collected.The expressions of miR-338-3p and the expressions of RAB23 mRNA in TC tissues and adjacent tissues were detected.The relationship between the expression of miR-338-3p and the expressions of RAB23 mRNA and the clinicopathological features of TC was analyzed.Kaplan-Meier method was used to analyze the survival of TC patients with high/low miR-338-3p and RAB23 mRNA expression.The influencing factors of poor prognosis in TC patients were analyzed by multivariate Cox regression.Results:Compared with adjacent tissues,the expression of miR-338-3p in TC tissues was decreased,and the expression of RAB23 mRNA was increased(P<0.05).The expression of miR-338-3p and RAB23mRNA was related to pathological type,TNM stage and lymph node metastasis(P<0.05).After 3 years of follow-up,the 3-year overall survival rate of 217 TC patients was 83.41%(181/217).The 3-year overall survival rate in miR-338-3p high expression group was higher than that in miR-338-3p low expression group,and the 3-year overall survival rate in RAB23 mRNA high expression group was lower than that in RAB23 mRNA low expression group(P<0.05).Multivariate Cox regression analysis showed that,miR-338-3p<0.62,RAB23mRNA≥2.13,lymph node metastasis,TNM stageⅢ~Ⅳand low-thyroid/undifferentiated carcinoma were independent risk factors for poor prognosis in TC patients.Conclusion:The low expression of miR-338-3p and the high expression of RAB23 mRNA in TC tissues are risk factors for poor prognosis of TC patients,TNM stage and lymph node metastasis of TC patients.