儿童乳酸与标本周转时间的分析

Relationship between Sample Turnaround Time and Pediatric Lactate Levels

目的:探讨标本存放时间与儿童血乳酸值的关系。方法:收集本院2022年10月的门诊及住院患儿的生化标本中乳酸结果、采样、签收和审核时间等数据,对门诊以及住院患儿的乳酸水平,周转时间以及住院患儿的采样时间点进行分析;随机采集12例患儿的血标本,置于4℃和室温下,于0分钟,15分钟,30分钟,60分钟,120分钟测定两组乳酸的增量随时间变化的关系。结果:共收集337例患儿的标本,发现住院患儿的乳酸结果显著高于门诊患儿的乳酸结果(2.10 mmol/L VS 1.50 mmol/L,P<0.001),住院患儿的采样与签收时间显著高于门诊患儿的时间(42.00分钟VS 10.00分钟,P<0.001)。住院患儿采集与签收的时间与星期无关,而在6:00-7:59的时间显著高于其他时间点。乳酸随着标本存放时间的延长以及温度的升高而增加,低温存放影响不明显,室温下影响更显著。结论:标本放置时间对乳酸的结果影响显著,需要加强采样到审核的流程管理,力求最短时间内检测乳酸标本。

Objective:To investigate the relationship between sample storage time and lactate levels in pediatric blood samples.Methods:Lactate results,sampling times,times of receipt,and review times were collected from biochemistry samples of outpatients and inpatients at our hospital in October 2022.Lactate levels,turnaround times and timing of sample collection were registered and analyzed for outpatients and inpatients.To further examine the relationship between sample storage time and pediatric blood lactate levels,12pediatric blood samples were selected randomly.Samples were stored at 4℃and room temperature.Lactate levels were measured at 0,15,30,60 and 120 minutes to analyze the incremental change in lactate over time.Results:A total of 337 pediatric samples were collected.Lactate levels were found to be significantly higher in inpatient samples compared to outpatient samples(2.10 mmol/L vs 1.50mmol/L,P<0.001).Additionally,the time between sampling and receipt at the laboratory was significantly longer for inpatient samples compared to outpatient samples(42.00 minutes vs 10.00 minutes,P<0.001).The time between sample collection and receipt for inpatients was not associated with day of the week.However,this time was significantly higher for samples collected between 6:00-7:59compared to other collection times.Lactate levels increased with longer storage time and higher temperature.The effect of refrigerated storage was unclear,while ambient temperature storage had a more significant effect on lactate levels.Conclusions:The study demonstrates that sample storage time significantly influences lactate level measurements.It is necessary to improve the process management from sampling to review,and strive to detect lactic acid specimens in the shortest time.