dnmt3b基因多态性与小儿HBV易感性及持续性感染的关系

Association of dnmt3b gene polymorphism with HBV susceptibility and persistent infection in children

目的探究DNA甲基转移酶3B(dnmt3b)基因多态性与小儿乙型肝炎病毒(HBV)易感性及持续性感染的关系,为预防和治疗乙肝提供新的依据和策略。方法选取中大惠亚医院2020年6月—2023年2月收治的HBV感染患儿104例,另选取院内同期健康体检儿童104名作为健康对照,采用PCR和DNA测序技术检测dnmt3b基因多态性。根据患儿治疗6个月内结局分为持续性感染组和感染后转阴组,分析持续性感染的危险因素。结果104例HBV感染患儿经抗HBV治疗后78例患儿HBsAg转阴,余26例患儿HBsAg持续阳性。持续性感染组丙氨酸氨基转移酶(ALT)(76.51±19.35)U/L、天冬氨酸氨基转移酶(AST)(72.34±20.06)U/L、血清HBV DNA载量(124.61±11.07)×103/ml,均高于转阴组(P<0.05),持续性感染组dnmt3b基因rs1550117位点中GG基因频率(73.08%)高于转阴组(39.74%)(P<0.05);ALT、AST、血清HBV DNA载量、dnmt3b rs1550117 GG基因型是小儿HBV持续性感染的危险因素(P<0.05),HBV感染组dnmt3b rs1550117位点中GG基因频率(40.08%)高于健康对照组(20.19%)(P<0.05)。结论dnmt3b rs1550117基因与HBV患儿的易感性及持续性感染有关,且GG基因型HBV患儿易感性的风险更高。

Objective To explore the relationship between DNA methyltransferase 3B(dnmt3b)gene polymorphism and the susceptibility and persistent infection of hepatitis B virus(HBV)in children,to provide novel basis and strategy for the prevention and treatment of hepatitis B.Methods A total of 104 children with HBV infection admitted to CUHK Huiya Hospital from June 2020 to February 2023 were selected,and 104 healthy children who underwent physical examination in the same hospital during the same period were selected as healthy control.PCR and DNA sequencing were used to detect the polymorphism of dnmt3b gene.According to the outcome within 6 months after treatment,the children were divided into persistent infection group and negative conversion group,the risk factors for persistent HBV infection were analyzed.Results Among 104 children with HBV infection,HBsAg turned negative in 78 children and remained positive in 26 children after anti-HBV treatment.The levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST)and serum HBV DNA load in persistent infection group were(76.51±19.35)U/L,(72.34±20.06)U/L,(124.61±11.07)×103/ml were higher than those in the negative conversion group(P<0.05).The frequency of GG gene in dnmt3b rs1550117 in the persistent infection group(73.08%)was higher than that in the negative conversion group(39.74%)(P<0.05).ALT,AST,HBV DNA load,and dnmt3b rs1550117 GG genotype were the risk factors for persistent HBV infection in children with hepatitis B(P<0.05).The frequency of GG gene in dnmt3b rs1550117 in the HBV infection group(40.08%)was higher than that in the healthy control group(20.19%)(P<0.05).Conclusion Dnmt3b rs1550117 gene was associated with susceptibility and persistent infection in HBV infection children,and GG genotype HBV infection children had a higher risk of susceptibility.