芍药花提取物中黄酮的测定及其体外抗氧化和降脂活性研究

Determination of flavonoids in Paeonia lactiflora flower extracts and their in vitro antioxidant and hypolipidemic activities

对芍药花不同溶剂提取物中黄酮含量、体外抗氧化活性以及降脂活性进行对比研究。本文采用NaNO2-Al(NO3)3显色法测定黄酮含量,以DPPH自由基清除率、ABTS自由基清除率和总还原能力为考察指标评价芍药花提取物的抗氧化能力,以抑制胰脂肪酶能力、结合胆酸盐能力及胆固醇吸附能力为考察指标评价芍药花提取物的降脂活性。结果表明:低共熔溶剂提取物总黄酮含量高于乙醇提取物总黄酮含量,同时低共熔溶剂提取法也提高了芍药花提取物的抗氧化活性和降脂活性,低共熔溶剂提取物对ABTS自由基清除的IC50值为0.256 mg/mL,对DPPH自由基清除的IC50值为0.296 mg/mL;对胰脂肪酶半数抑制浓度为0.788 mg/mL,对胆酸钠、牛磺胆酸钠和甘氨酸钠结合的IC50值分别为0.883、0.841、0.850 mg/mL,在芍药花粗提物用量为0.5 g时,对胆固醇吸附量最大为6.28 mg/g。因此低共熔溶剂可作为提取芍药花中抗氧化和降脂成分的适宜提取溶剂,提取物具有较好的抗氧化和降脂潜力,为芍药花后续高价值化利用提供理论基础。

This paper presents a comparative study of flavonoid content,in vitro antioxidant activity and lipid-lowering activity of different solvent extracts of Paeonia lactiflora flower.In this paper,the flavonoid content was determined by NaNO 2-Al(NO 3)3 colorimetric method,the antioxidant capacity of P.lactiflora flower extract was evaluated by DPPH radical scavenging rate,ABTS radical scavenging rate,and total reducing capacity,and the lipid-lowering activity of P.lactiflora flower extract was evaluated by pancreatic lipase inhibition capacity,bile-acid-binding capacity,and cholesterol-adsorption capacity.The results showed that the total flavonoid content of the low-eutectic solvent extract was higher than that of the ethanol extract,and also the low-eutectic solvent extraction method improved the antioxidant activity and lipid-lowering activity of P.lactiflora flower extracts,and the IC 50 values of the low-eutectic solvent extracts for the scavenging of ABTS free radicals were 0.256 mg/mL,and for the scavenging of DPPH free radicals,the IC 50 values of the low-eutectic solvent extracts for the scavenging of DPPH free radicals were 0.296 mg/mL;and the IC 50 values for the inhibition of pancreatic Lipase half inhibitory concentration was 0.788 mg/mL,the IC 50 values for sodium cholate,sodium taurocholate and sodium glycinate binding were 0.883,0.841,0.850 mg/mL,respectively,and the maximum adsorption of cholesterol was 6.28 mg/g when the dosage of P.lactiflora flower crude extract was 0.5 g.Therefore,low eutectic solvent can be used as a suitable extraction solvent for extracting antioxidant and lipid-lowering components from P.lactiflora flower,and the extracts have better antioxidant and lipid-lowering potentials,which can provide theoretical basis for the subsequent high-value utilization of P.lactiflora flower.