基于广泛靶向代谢组学分析红花羊蹄甲不同部位挥发性成分差异

Differential analysis of volatile components in different parts of Bauhinia blakeana by using widely-targeted matabolomics

红花羊蹄甲(Bauhinia blakeana Dunn.)兼具园林观赏及药用价值。为进一步探究红花羊蹄甲不同部位挥发性成分的差异,利用气相色谱-质谱(GC-MS)广泛靶向代谢组学技术鉴定并分析叶片与花朵的挥发性成分差异。结果表明,在叶片和花朵中共鉴定到840个挥发性成分,分为萜类、酯、杂环化合物等16类化合物。差异代谢物在倍半萜和三萜生物合成通路中显著富集。PCA结果表明,红花羊蹄甲不同部位的挥发性成分之间存在差异。聚类热图结果表明,大多数代谢物在花朵中的富集量相对较高。不同部位显著差异代谢物有90种,红花羊蹄甲叶片中代谢物种类含量较高的是酮、醛类;花朵中代谢物种类含量较高的是萜类、酯类。本研究通过对红花羊蹄甲不同部位中的挥发性成分进行全面分析和鉴定,为后续深入挖掘和利用红花羊蹄甲中多样化的挥发性成分提供理论依据。

Bauhinia blakeana Dunn.has both ornamental and medicinal value.In order to further explore the volatile metabolites in different parts of B.blakeana,gas chromatography-mass spectrometry(GC-MS)was used to identify and analyze the differences of metabolites between leaves and flowers.The results showed that a total of 840 volatile metabolites were identified in leaves and flowers,which were divided into 16 compounds,such as terpenoids,esters,heterocyclic compounds and so on.Differential metabolites were significantly enriched in sesquiterpene and triterpene biosynthesis pathways.The results of principal component analysis showed that there were differences in volatile metabolites between different parts of B.blakeana.The results of cluster heat map showed that the enrichment of most metabolites in flowers was relatively high.There were 90 kinds of metabolites with significant differences in different parts.In the leaves of B.blakeana,the higher contents of metabolite types were ketones and aldehydes,while there were terpenoids and esters in the flowers.In this study,the metabolite types in different parts of B.blakeana were comprehensively analyzed and identified,which provided a theoretical basis for the further exploration and utilization of the various of volatile components in B.blakeana.