和田玫瑰精油提取工艺优化及其抗氧化和美白活性研究

Optimization of extraction technology of essential oil of Rosa rugosa Hetian and its antioxidant and whitening activities

探究和田玫瑰精油的最佳提取工艺以及其抗氧化和美白活性。以和田玫瑰为原料,采用盐浸辅助水蒸气蒸馏法提取玫瑰精油,研究盐浸浸泡时间、NaCl质量浓度和料液比对精油提取量的影响,在单因素试验的基础上采用Box-Behnken响应面法优化提取工艺参数,最优提取条件得到的和田玫瑰精油进行体外和细胞水平实验来验证其抗氧化和美白活性。结果表明:在盐浸浸泡时间为2.5 h,NaCl质量浓度为5.4%,料液比1∶7.3(g/mL)条件下精油提取量最高,可达到23.35 mg/g(干花)。在此条件下得到的玫瑰精油对DPPH自由基清除能力的IC50值为7.14 mg/mL;细胞实验结果表明,和田玫瑰精油能降低α-MSH诱导后B16F10细胞中的黑色素含量,抑制细胞水平上酪氨酸酶的活性。本研究提供了一种高效提取和田玫瑰精油的方法,证明了和田玫瑰精油具有一定的抗氧化和美白活性,为和田玫瑰精深加工以及抗氧化美白应用方面提供了科学依据。

In order to explore the optimal extraction process of the Rosa rugosa Hetian essential oil and its antioxidant and whitening activities,this experiment used rose essential oil as raw material,which was extracted by salt-soaking assisted steam distillation.On the basis of single factor experiment,the effects of salt-soaking time,NaCl mass concentration,and solid-liquid ratio on essential oil extraction were studied,and Box-Behnken response surface method was used to optimize the extraction process parameters.Then,in vitro and cellular level experiments were conducted to verify the antioxidant and whitening activities of rose essential oil obtained from the optimal extraction conditions.The results showed that the extraction amount of essential oil was the highest,which could reach 23.35 mg/g(dried flowers)under the conditions of salt soaking time of 2.5 h,NaCl concentration of 5.4%and material-liquid ratio of 1∶7.3(g/mL).Under these conditions,the IC 50 value of scavenging DPPH radical of rose essential oil was 7.14 mg/mL.The results of cell experiments showed that the R.rugosa Hetian essential oil could reduce the melanin content and inhibit the tyrosinase activity in theα-MSH induced B16F10 cells.To sum up,this study provides an efficient method to extract the R.rugosa Hetian essential oil,which proves that rose essential oil has certain antioxidant and whitening activities,further provides a scientific basis for the deep processing,antioxidant and whitening application of the R.rugosa Hetian essential oil.