超声辅助低共熔溶剂提取韩信草总黄酮的工艺优化

Optimization of extraction technology of total flavonoids from Scutellariae Indicae Herba with ultrasonic-assisted deep eutectic solvents

为提高韩信草中总黄酮的提取率,实验采用超声辅助低共熔溶剂——氯化胆碱-乙二醇提取韩信草中的总黄酮。首先采用冷冻干燥法合成氯化胆碱-乙二醇低共熔溶剂,以单因素试验确定含水率、料液比、超声功率、超声时间对韩信草中总黄酮提取量的影响,通过Plackett-Burman法结合Box-Behnken响应面法进一步对超声提取工艺进行优化。试验结果表明,该提取工艺的最佳条件为含水率59.30%,液料比25.90∶1,超声功率121 W,总黄酮含量为7.924 mg/g。相同条件下采用氯化胆碱-乙二醇为提取溶剂明显高于其他传统溶剂,可实现总黄酮高效提取的优点。因此,通过优化后的超声辅助低共熔溶剂氯化胆碱-乙二醇法能明显提高总黄酮的提取率。

To enhance the extracting amount of flavonoids in Scutellariae Indicae Herba,an experiment employed deep eutectic solvents(DESs)choline chloride-ethylene glycol approach to extract flavonoids from Scutellariae Indicae Herba.The extracting process was optimized through a combination of single factor experiment and Plackett-Burman design along with Box-Behnken response surface methodology,and the influential factors such as moisture content,liquid-solid ratio,and ultrasound power were utilized to assess the extraction yield of total flavonoids in Scutellariae Indicae Herba.The results demonstrated that the optimal extracting conditions were a moisture content of 59.30%,a liquid-solid ratio of 25.90∶1,and an ultrasound power of 121 W,with amount of flavonoids 7.924 mg/g.Under the same circumstances,the extracting efficiency of flavonoids using choline chloride-ethylene glycol as the extraction solvent was significantly higher than that of other conventional solvents,and the advantage of efficient extracting amount of flavonoids was attained.Hence,the optimized ultrasound-assisted deep eutectic solvents choline chloride-ethylene glycol method can conspicuously improve the extracting amount of flavonoids.