基于LC-MS分析白僵菌侵染家蚕过程中主要代谢物的变化

Changes of major metabolites in the process of Beauveria bassiana infecting Bombyx mori based on LC-MS Analysis

利用LC-MS分析方法,从代谢组学角度分析白僵菌Beauveria bassiveria侵染家蚕Bombyx mori幼虫过程中的代谢物种类变化,基于KEGG Pathway数据库对差异代谢物进行富集分析。结果表明:在白僵菌孢子萌发前期,主要产生荜茇酰胺、地奥司明、juglalin等40种代谢物,以黄酮类、生物碱为主;在白僵菌芽管期形成I期,主要产生苦杏仁苷、isorhamnetin-3-glucoside、生物素、烟酸等39种代谢物,以有机酸、糖苷类、维生素类为主;在白僵菌芽管期形成II期,主要产生myristoleic acid、ephedrine、verruculogen、阿霉素、D-glycero-D-galacto-heptitol等100种代谢物,以有机酸、生物碱、抗生素类为主;在白僵菌附着胞形成期,主要产生neoglucobrassicin、2-aminoisobutyric acid等21种代谢物,以糖苷类、氨基酸类为主。与孢子萌发前期相比,白僵菌到芽管期形成I期,有138种代谢物显著上调,主要是有机酸类、氨基酸类、酯类,97种代谢物显著下调,主要是有机酸类、醇类、胺类;与芽管期形成I期相比,到芽管期形成II期,有136种代谢物显著上调,主要是有机酸类、胺类、核苷类,60种代谢物显著下调,主要是有机酸类、酯类、氨基酸类;与芽管期形成II期相比,到附着胞形成期,有52种代谢物显著上调,主要是有机酸类、氨基酸类、酰胺类、酚类、糖类,242种代谢物显著下调,主要是有机酸类、酯类、酚类、氨基酸类、酰胺类、核苷类。在白僵菌侵染过程中,差异代谢物主要参与的代谢通路有FoxO signaling pathway、mTOR signaling pathway、local analgesics等,且到芽管期形成期,差异代谢物参与的代谢通路较多。该研究可为揭示白僵菌对寄主昆虫的侵染机制提供理论参考。

LC-MS analysis method was used to analyze the changes of metabolites during infection of Bombyx mori by Beauveria bassiveria from the perspective of metabolomics,and the enrichment analysis of differential metabolites was conducted based on KEGG Pathway database.In the early stage of spore germination of B.bassiveria,40 metabolites were mainly produced,such as piplartine,diosmin and juglalin,etc.,mainly flavonoids and alkaloids.In the stage I of germ tube formation of B.bassiana,39 metabolites were mainly produced,such as amygdalin,isorhamnetin-3-glucoside,biotin and nicotinic acid,etc.,mainly organic acids,glycosides and vitamins.In the stage II of germ tube formation of B.bassiana,100 metabolites were mainly produced,such as myristoleic acid,ephedrine,verruculogen,doxorubicin,D-glycero-D-galacto-heptitol,etc.,mainly organic acids,alkaloids,antibiotics.In the stage of appressorium formation of B.bassiana,21 metabolites were mainly produced,such as neoglucobrassicin and 2-aminoisobutyric acid,etc.,mainly glycosides and amino acids.Compared with the early stage of spore germination,138 metabolites were significantly up‑regulated by the stage I of germ tube formation,mainly organic acids,amino acids and esters,and 97 metabolites were significantly down‑regulated,mainly organic acids,alcohols and amines.Compared with the stage I of germ tube formation,136 metabolites were significantly up‑regulated by the stage II of germ tube formation,mainly organic acids,amines and nucleosides,while 60 metabolites were significantly down‑regulated,mainly organic acids,esters and amino acids.Compared with the stage II of germ tube formation,52 metabolites were significantly up‑regulated by appressorium formation stage,mainly organic acids,amino acids,amides,phenols and carbohydrate,and 242 metabolites were significantly down‑regulated,mainly organic acids,esters,phenols,amino acid,amides and nucleoside.During the infection process of B.bassiana,the differential metabolites were mainly involved in FoxO signaling pathway,mTOR signaling pathway,local analgesics,etc.,and to the stage of the germ tube formation,the differential metabolites were involved in many metabolic pathways.This study can provide theoretical reference for revealing the infection mechanism of B.bassiana on host insects.