RP-HPLC法快速测定常山胡柚果皮中3种主要成分

Rapid determination of three main components in the peel of Changshan-huyou by RP-HPLC

目的建立常山胡柚果皮中3种主要成分的含量测定方法。方法采用RP-HPLC法测定含常山胡柚果皮中芸香柚皮苷、柚皮苷和新橙皮苷的含量,色谱柱为安捷伦ZORBAX Eclipse Plus C_(18)(4.6×250mm,5μm),流动相为甲醇(A)-0.1%乙酸-水溶液(B),流速为1.0mL·min^(-1),于283nm波长下检测,柱温35℃,进样量5μL。结果常山胡柚果皮中芸香柚皮苷、柚皮苷和新橙皮苷在一定范围内线性良好(r>0.9992)。结论该法操作简便,灵敏度高,检测快速,适用于常山胡柚果皮中黄酮类成分的含量测定。

OBJECTIVE To establish a method for the determination of three main components in the peel of Changshan-huyou.METHODS The contents of narirutin,naringin and neohesperidin in the peel of Changshan-huyou were determined by RP-HPLC,HPLC analysis was performed on a Agilent ZORBAX Eclipse Plus C_(18)(4.6×250mm,5μm),the mobile phase was methanol(A)-0.1%Acetic acid-water solution(B)at a flowrate of 1.0mL·min^(-1),and the detection wave lengths were set at 283nm,the column temperature was 35℃,and injection volume was 5μL.RESLUTS The linearity of narirutin,naringin and neohesperidin in the peel of Changshan-huyou was weel in a certain range(r>0.9992).CONCLUSION The method has the advantages of simple operation,high sensitivity and rapid detection,and is suitable for the determination of flavonoids in the peel of Changshan-huyou.