瑶药白面风多酚提取工艺优化及其抗氧化和抗菌活性研究

Polyphenols Extraction Process Optimization and Research Antibacterial and Antioxidant Activity from Lnula cappa(Buch.-Ham.)DC

研究旨在探究微波辅助提取白面风多酚优化工艺及其抗氧化和抗菌活性。试验在单因素试验的基础上采用响应面法进行了白面风多酚提取工艺的优化,并以DPPH·、·OH清除率为指标探究白面风多酚抗氧活化性,以抑菌圈直径和最低抑菌浓度为指标探究白面风多酚抗菌活性。结果表明,白面风多酚最佳提取工艺参数为:微波时间5.3 min,乙醇体积分数56%,料液比1∶42 g/mL,微波功率294 W,此条件下多酚得率为(0.9984±0.0033)%;白面风多酚对DPPH·、·OH有较好的清除力,当多酚浓度分别为0.7 mg/mL和0.89 mg/mL时,对DPPH·和·OH清除率分别为(86.34±1.52)%和(70.27±0.93)%;当白面风多酚浓度为100 mg/mL时,其对大肠杆菌和金黄色葡萄球菌的抑菌直径分别(7.50±0.50)mm和(8.00±0.63)mm,最低抑菌浓度分别为3.70 mg/mL和11.11 mg/mL。瑶药白面风多酚具有抗氧化和抗菌双重活性,具有一定的饲料添加剂开发潜力。

The aim of this study was to explore the microwave-assisted extraction of Lnula cappa(Buch.-Ham.)DC polyphenols and its antioxidant and antibacterial activities.This study used response surface methodology to optimize the extraction process of Lnula cappa(Buch.-Ham.)DC polyphenols based on single factor experiments,and explored the antioxidant activity of Lnula cappa(Buch.-Ham.)DC polyphenols using DPPH·and·OH clearance rates as indicators,and the antibacterial activity of Lnula cappa DC polyphenols using the diameter of the antibacterial zone as indicators and minimum inhibitory concentration.The results showed that the optimum conditions for extraction L.cappa(Buch.-Ham.)DC polyphenols were as follows:microwave time 5.3 min,ethanol volume fraction 55%,solid-liquid ratio 1∶42 g/mL,microwave power 294 W.Under these conditions,the extraction rate of polyphenols was(0.9984±0.0033)%.The DPPH·and·OH scavenging abilities of L.cappa(Buch.-Ham.)DC polyphenols were good.When the concentration of polyphenols was 0.7 mg/mL and 0.89 mg/mL,the scavenging rates of DPPH·and·OH were(86.34±1.52)%and(70.27±0.93)%respectively.In addition,when the concentration of polyphenols was 100 mg/mL,the inhibitory diameters against Escherichia coli and Staphylococcus aureus were(7.50±0.50)mm and(8.00±0.63)mm,respectively,and the minimum inhibitory concentration against the two bacteria were 3.70 mg/mL and 11.11 mg/mL,respectively.The polyphenols from Yao medicine L.cappa(Buch.-Ham.)DC.showed bot antioxidant and antibacterial activities,and have some potential for the development of feed additives.