核桃青皮多糖对肠道致病菌抑菌活性研究

Study on Antibacterial Activities of Green Walnut Husk Polysaccharides on Intestinal Pathogenic Bacteria

为探究核桃青皮多糖(GWHP)对肠道致病菌的抑菌活性,以大肠埃希氏菌(Escherichia coli)、金黄色葡萄球菌(Staphylococcus aureus)和艰难梭菌(Clostridium difficile)为靶向菌种,通过测定抑菌圈、最小抑菌浓度(MIC)和生长曲线分析核桃青皮多糖对肠道致病菌的抑菌活性。结果表明,3种核桃青皮多糖组分(GWHP-1、GWHP-2和GWHP-3)对大肠埃希氏菌、金黄色葡萄球菌和艰难梭菌均有抑制作用,且对3种致病菌的生长曲线均有影响。GWHP-1的抑菌作用最强,其对大肠埃希氏菌、金黄色葡萄球菌和艰难梭菌的MIC值分别为1.00、2.00和4.00 mg/mL;GWHP-2次之,其对3种致病菌的MIC值分别为4.00、4.00和16.00 mg/mL;GWHP-3的抑菌作用最弱,其对3种致病菌的MIC值分别为8.00、16.00和16.00 mg/mL。核桃青皮多糖有良好的抑菌活性,具有被开发成抑菌保鲜剂的潜力。

To explore antibacterial activities of green walnut husk polysaccharides(GWHP)on intestinal pathogenic bacteria,taking Escherichia coli,Staphylococcus aureus and Clostridioides difficile as targeted bacterial species,antibacterial activities of GWHP on intestinal pathogenic bacteria were analyzed by measuring inhibition zones,minimum inhibitory concentrations(MIC)and growth curves.Results showed that three components of GWHP(GWHP-1,GWHP-2 and GWHP-3)could inhibit E.coli,S.aureus and C.difficile,and had effects on growth curves of three pathogenic bacteria.GWHP-1 had the strongest inhibitory effects with MIC values for E.coli,S.aureus and C.difficile being 1.00,2.00 and 4.00 mg/mL respectively.Followed by GWHP-2 with MIC values for three pathogenic bacteria being 4.00,4.00 and 16.00 mg/mL respectively.GWHP-3 had the weakest inhibitory effects with MIC values for three pathogenic bacteria being 8.00,16.00 and 16.00 mg/mL respectively.GWHP had good antibacterial activities and had potentials to be developed as preservatives.