响应面法优化云南华坪仙人掌多糖提取工艺及抗癌活性研究

Optimization of Extraction Process of Polysaccharidefrom Yunnan Huaping Cactus Gum by Response SurfaceMethodology and the Anticancer Activity Research

(目的)探讨仙人掌中多糖的提取工艺和抗癌活性。(方法)利用单因素实验和响应面法优化了仙人掌粗多糖的提取工艺,并利用MTT法评价该多糖的抗癌活性。(结果)经响应面法优化得到的仙人掌粗多糖的最佳提取工艺为:提取时间5 h、提取次数5次、料液比1∶60 g/mL、乙醇体积分数70%,仙人掌粗多糖提取率为87.02%。经响应面预测得到的最佳工艺与实验条件最佳条件相同,经进一步验证提取率为(82.38±3.19)%。MTT法测定仙人掌粗多糖抑制肿瘤细胞生长活性的结果表明,仙人掌粗多糖对结肠癌细胞(RKO)和乳腺癌细胞(4T1)有一定的抑制活性,IC_(50)值分别为143.5和167.9 mg/L。(结论)本研究获得的工艺条件能有效的提取仙人掌胶中的多糖,且得率高;活性实验表明仙人掌胶多糖对结肠癌细胞(RKO)和乳腺癌细胞(4T1)有一定的抑制活性。

(Objective)To explore the processing parameters of the cactus gum polysaccharides and the antitumor activity.(Methods)This study optimized the extraction process of crude polysaccharides from cactus gum using single-factor experiment and response surface methodology(RSM),and evaluated the anticancer activity of the polysaccharides using the MTT assay.(Results)Firstly,the single factor experiments and response surface methodology(RSM)were used to optimize the extraction process of crude polysaccharides from cactus gum.The optimum extraction conditions for crude cactus gum polysaccharides by RSM is as follows:extraction time 5 hours,extraction 5 times,the liquid ratio was 1∶60(g/mL)and ethanol concentration was (70%V/V).Under this condition,the extraction rate of crude polysaccharides in cactus gum is 87.02%.Next,the optimal conditions predicted by RSM is consistent with experimental conditions,and further verification shows that the extraction rate is(82.38±3.19)%.Finally,the MTT assay was employed to evaluate the anti-tumor cell growth activity by the cactus gum polysaccharide.The results showed that cactus gum polysaccharides had certain inhibitory activity on colon cancer cells(RKO)and breast cancer cells(4T1),IC_(50) values were 143.5 and 167.9 mg/L,respectively.(Conclusion)The extraction conditions of the cactus gum polysaccharides can effectively extract polysaccharides with high yield in this study.It was found that cactus gum polysaccharides can inhibit the growth of colon cancer cells(RKO)and breast cancer cells(4T1).